Self Paced

CRISPR-Cas Genome Editing: Workflow, Tools and Techniques

Empower Your Future with Precision Genome Editing: Master the CRISPR-Cas9 Technology

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Online/ e-LMS
Self Paced
1 Month


CRISPR-Cas9 has transformed the landscape of genetic engineering, offering unprecedented precision and efficiency in genome editing. This one-month program delves into the foundational principles of CRISPR-Cas9, exploring its historical development, molecular mechanisms, and the wide array of tools and techniques utilized in genome editing. Participants will gain a comprehensive understanding of the entire workflow, from designing guide RNAs to analyzing edited cells.

Throughout the program, learners will engage in practical sessions, hands-on experiments, and real-world case studies, allowing them to apply theoretical knowledge in practical scenarios. The curriculum also covers ethical considerations, regulatory frameworks, and the potential impact of genome editing on various sectors, including biotechnology, agriculture, and medicine. By the end of the program, participants will be equipped with the skills and knowledge to pursue careers in genetic engineering and related fields, driving innovation and contributing to significant scientific advancements.


The aim of the “CRISPR-Cas Genome Editing: Workflow, Tools and Techniques” program is to provide participants with a thorough understanding and practical experience of CRISPR-Cas9 technology. This program aims to equip learners with the necessary skills to utilize CRISPR-Cas9 for various genetic engineering applications, enabling them to contribute effectively to advancements in biotechnology, medicine, and agriculture.

Program Objectives

  • Understand the molecular mechanisms and principles of CRISPR-Cas9 genome editing.
  • Gain proficiency in designing and implementing CRISPR-Cas9 experiments.
  • Explore the ethical and regulatory aspects of genome editing.
  • Apply CRISPR-Cas9 technology to real-world problems in various industries.
  • Develop critical thinking and problem-solving skills in genetic engineering.

Program Structure

Module 1: Introduction and applications of CRISPR-Cas

  • What is CRISPR-Cas?
  • How does CRISPR-Cas work?
  • Different types of CRISPR-Cas systems
  • CRISPR in Basic Research
  • CRISPR in Translational Research
  • CRISPR in Gene therapy

Module 2: CRISPR-Cas gene editing workflows and challenges and troubleshooting

  • Designing a CRISPR-Cas experiment
  • Assembling CRISPR-Cas components
  • Transfecting or transducing cells with CRISPR-Cas components
  • Validating gene editing results
  • Off-target effects
  • Low efficiency
  • Delivery challenges

Module 3: Advanced CRISPR-Cas gene editing techniques, ethics and regulation

  • CRISPR-Cas gene activation and repression
  • CRISPR-Cas gene editing in primary cells and tissues
  • CRISPR-Cas genome engineering for animal models
  • Responsible use of CRISPR-Cas
  • Intellectual property considerations
  • Regulatory landscape for CRISPR-Cas gene editing

Participant’s Eligibility

  • Undergraduate degree in Molecular Biology, Genetics, Biotechnology, or related fields.
  • Professionals in the biotechnology, pharmaceutical, or agricultural industries.
  • Researchers and scientists with a keen interest in genetic engineering and genome editing technologies.

Program Outcomes

  • Mastery of CRISPR-Cas9 genome editing techniques.
  • Ability to design and execute genome editing experiments.
  • In-depth understanding of ethical and regulatory considerations.
  • Proficiency in analyzing and interpreting genetic data.
  • Preparedness for careers in genetic engineering and biotechnology.

Fee Structure

Standard Fee:           INR 4,998           USD 110

Discounted Fee:       INR 2499             USD 55





Program Assessment

Certification to this program will be based on the evaluation of following assignment (s)/ examinations:

Exam Weightage
Mid Term Assignments 20 %
Final Online Exam 30 %
Project Report Submission (Includes Mandatory Paper Publication) 50 %

To study the printed/online course material, submit and clear, the mid term assignments, project work/research study (in completion of project work/research study, a final report must be submitted) and the online examination, you are allotted a 1-month period. You will be awarded a certificate, only after successful completion/ and clearance of all the aforesaid assignment(s) and examinations.

Program Deliverables

  • Access to e-LMS
  • Real Time Project for Dissertation
  • Project Guidance
  • Paper Publication Opportunity
  • Self Assessment
  • Final Examination
  • e-Certification
  • e-Marksheet

Future Career Prospects

  • Genetic Engineer
  • Biomedical Scientist
  • Biotechnology Researcher
  • Agricultural Biotechnologist
  • Clinical Research Scientist
  • Regulatory Affairs Specialist

Enter the Hall of Fame!

Take your research to the next level!

Publication Opportunity
Potentially earn a place in our coveted Hall of Fame.

Centre of Excellence
Join the esteemed Centre of Excellence.

Networking and Learning
Network with industry leaders, access ongoing learning opportunities.

Hall of Fame
Get your groundbreaking work considered for publication in a prestigious Open Access Journal (worth ₹20,000/USD 1,000).

Achieve excellence and solidify your reputation among the elite!


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