CRISPR-Cas Genome Editing: Mechanisms and Applications

Mastering Gene Editing: Unlock the Potential of CRISPR

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MODE
Online/ e-LMS

TYPE
Self Paced

LEVEL
Moderate

DURATION
3 Months

About

The CRISPR-Cas Defense Mechanism program delves into the revolutionary gene-editing technology that has transformed scientific research and biotechnology. Over the course of one month, students will learn about different CRISPR-Cas systems, their discovery, and how they can be harnessed for genome editing across various organisms. The curriculum is designed to cover both theoretical concepts and practical applications, including lab simulations and case studies of CRISPR in therapeutic settings and agriculture.

Aim

This program aims to provide an in-depth understanding of the CRISPR-Cas systems, highlighting their roles in microbial immunity and biotechnological applications. Participants will explore the molecular mechanisms, ethical implications, and the latest advancements in CRISPR technology to prepare for careers in genetic engineering and biomedicine.

Program Objectives

Understand the fundamentals of CRISPR-Cas systems and their biological functions.
Gain hands-on experience with CRISPR design and applications in lab simulations.
Explore the ethical and regulatory frameworks governing gene editing.
Analyze case studies on the impact of CRISPR technologies in medicine and agriculture.
Prepare for advanced studies or careers in genetic editing technologies.

Program Structure

Month 1: Foundations of CRISPR-Cas Technology

Week 1: Introduction to Genome Editing

Historical perspective of genome editing
Overview of genome editing technologies
Discovery and development of CRISPR-Cas systems

Week 2: Molecular Mechanisms of CRISPR-Cas

Structure and function of CRISPR-Cas components
Mechanism of CRISPR-Cas action
Types of CRISPR-Cas systems (Class 1, Class 2)

Week 3: CRISPR-Cas in Prokaryotes

Adaptive immunity in bacteria and archaea
Natural functions and diversity of CRISPR-Cas systems
Biogenesis and interference phases

Week 4: Guide RNA Design and Optimization

Principles of guide RNA design
Bioinformatics tools for target site selection
Strategies to enhance specificity and efficiency

Month 2: Practical Applications and Methodologies

Week 1: CRISPR-Cas in Genetic Engineering

Gene knockout and knock-in techniques
Base editing and prime editing
Applications in model organisms

Week 2: Delivery Methods for CRISPR-Cas

Delivery vectors (plasmids, viral vectors)
Non-viral delivery methods (electroporation, nanoparticles)
Tissue-specific delivery approaches

Week 3: CRISPR-Cas for Disease Modeling

Generation of disease models using CRISPR-Cas
Applications in studying genetic diseases
High-throughput screening using CRISPR libraries

Week 4: CRISPR-Cas in Therapeutics

Clinical applications and gene therapy
Strategies for in vivo and ex vivo editing
Ethical considerations and regulatory aspects

Month 3: Advanced Applications and Future Directions

Week 1: Agricultural Biotechnology and CRISPR

Genome editing in plants and crops
Enhancing crop yield and resistance
Case studies in agricultural applications

Week 2: CRISPR-Cas in Synthetic Biology

Engineering synthetic gene networks
Applications in metabolic engineering
CRISPR-based biosensors

Week 3: Emerging Technologies and Innovations

Next-generation CRISPR technologies
High-throughput CRISPR screening methods
Multiplexed genome editing

Week 4: Case Studies and Future Prospects

Analysis of recent CRISPR-Cas research papers
Future directions in CRISPR-Cas research
Career opportunities in genome editing

Intended For

Undergraduate degree in Molecular Biology, Biotechnology, Genetics, or related fields.
Professionals in biomedical research, pharmaceuticals, or agricultural biotechnology.
Individuals with a keen interest in genetic engineering and genome editing technologies.

Program Outcomes

Proficiency in CRISPR-Cas technology applications
Understanding of molecular techniques in genetic engineering
Ability to address ethical considerations in biotech
Readiness for advanced research or professional roles
Skills in interdisciplinary collaboration and innovation

Fee Structure

Standard Fee: INR ~~14,998~~ USD ~~258~~

Discounted Fee: INR 7499 USD 129

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