Microbiome Bioinformatics: 16S, Biomarkers & Precision Probiotics
From Raw 16S Data to Microbiome Biomarkers and Precision Probiotic Candidates
About This Course
Microbiome research is rapidly moving beyond basic taxonomic profiling toward functional interpretation, biomarker discovery, and precision microbiome applications.
This 3-day practical workshop takes participants through the complete workflow from NCBI-SRA raw 16S sequencing data to ASV generation, microbial diversity analysis, functional profiling, biomarker identification, and probiotic candidate prioritization.
Aim
To provide participants with a practical understanding of 16S microbiome bioinformatics, from raw sequencing data processing to functional profiling, biomarker discovery, and precision probiotic candidate prioritization.
Workshop Objectives
- Process raw 16S sequencing data into ASV and taxonomy tables
- Perform alpha and beta diversity analysis
- Analyze multi-group microbiome datasets and clinical variables
- Apply differential abundance and functional profiling methods
- Use PICRUSt2 for pathway-level interpretation
- Identify and prioritize microbiome biomarkers
- Understand cross-study validation strategies
- Explore precision probiotic candidate discovery
Workshop Structure
Day 1 – From Raw 16S Data to Microbiome Profiles
- Introduction to 16S microbiome analysis
- Downloading raw sequencing data from NCBI-SRA
- FASTQ quality checking and preprocessing
- DADA2 workflow for ASV generation
- Taxonomic assignment
- Alpha and beta diversity analysis
- PCoA and microbial abundance visualization
Hands-On: Raw FASTQ → ASV Table → Taxonomy → Diversity Plots
Day 2 – Functional Profiling & Biomarker Discovery
- Differential abundance analysis
- Comparing multiple sample groups
- Working with clinical and experimental variables
- PICRUSt2 functional profiling
- Identifying important microbial features
- Introduction to microbiome biomarker discovery
- Random Forest and ROC/AUC for biomarker evaluation
Hands-On: ASV Table → Functional Profiles → Candidate Biomarkers
Day 3 – Biomarker Validation & Precision Probiotics
- Combining results from multiple microbiome studies
- Cross-study biomarker validation
- Linking microbial taxa with biological functions
- Identifying beneficial and depleted microbes
- Precision probiotic candidate prioritization
- Species-level vs strain-level interpretation
Hands-On: Validated Biomarkers → Functional Association → Probiotic Candidate Ranking
Who Should Enrol?
- MSc / MTech / Biotechnology students
- PhD scholars
- Postdoctoral researchers
- Bioinformatics researchers
- Microbiologists
- Biotechnology researchers
- Clinical researchers
- Computational biologists
- Faculty members
- Pharmaceutical and biotech professionals
- Researchers working in microbiome and probiotic science
Important Dates
Registration Ends
September 28, 2026
IST 4:30 PM
Workshop Dates
September 28, 2026 – September 30, 2026
IST 5:00 PM
Workshop Outcomes
- Convert raw 16S sequencing data into analysis-ready ASV tables
- Select and interpret appropriate microbiome diversity metrics
- Perform taxonomic and functional microbiome profiling
- Analyze multi-group microbiome datasets
- Identify statistically significant microbial features
- Discover and evaluate microbiome biomarkers
- Use PICRUSt2 for predicted functional pathway analysis
- Apply machine learning for biomarker ranking
- Perform cross-study biomarker validation
- Prioritize potential precision probiotic candidates
Recording fee
Participants in India
₹2,499
Participants outside India
$50
- All session recordings
- Study material
- Certificate after completion
- 1-year access
The live sessions of this workshop are over, so the live fees no longer apply. The recordings are being uploaded. They appear in your dashboard as soon as they are ready, and we will e-mail you then.
Frequently asked questions
Will I get the recording?
Yes. Paid participants get the session recordings and study material in their NanoSchool dashboard (My registrations → Recorded Lectures / Study Material).
What if I miss a live session?
Watch the recording in your dashboard and send your questions to the mentor through the support desk.
Do I get a certificate?
Yes, an e-certificate of participation after the workshop, with a QR code anyone can verify and an "Add to LinkedIn" button.
Which fee category applies to me?
Choose the category that matches your current role: Student, Ph.D. Scholar / Researcher, Academician / Faculty or Industry Professional.
How can I pay?
By card, UPI or PayPal on the payment page; international bank transfer is also available there.
What is the refund policy?
See our Returns, Refunds & Cancellation policy: Returns, Refunds & Cancellation policy