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Workshop Registration End Date :2024-12-11

Virtual Workshop

RNA Interference (RNAi) and siRNA Techniques

Master the Science of RNA Interference and siRNA Techniques to Transform Gene Therapy and Genetic Research

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MODE
Virtual (Google Meet)

TYPE
Mentor Based

LEVEL
Moderate

DURATION
3 Days (1.5 hours per day)

Start Date
11 – Dec – 24

Time
3:00 PM IST

About

RNA interference (RNAi) is a natural cellular process used to regulate gene expression. This 3-day workshop will delve into the fundamental mechanisms behind RNAi and its application in controlling the expression of specific genes. Participants will explore both theoretical and practical aspects of small interfering RNA (siRNA) techniques, from the molecular biology involved to real-world applications in research and medicine.

Throughout the workshop, we will focus on the role of RNAi in biological research, including gene function analysis, therapeutic approaches in diseases like cancer and viral infections, and gene editing techniques. The workshop will offer hands-on guidance in designing siRNA experiments, selecting appropriate delivery systems, and interpreting results, making it a valuable tool for molecular biologists, geneticists, and medical researchers.

Aim

This workshop aims to equip participants with a comprehensive understanding of RNA interference (RNAi) and small interfering RNA (siRNA) techniques. It will cover key mechanisms, applications in gene silencing, and how RNAi can be utilized in research and therapeutic strategies. By the end of the workshop, participants will be able to design and implement RNAi experiments for various biological studies.

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Workshop Objectives

Understand the mechanisms of RNA interference and its biological significance.
Learn about siRNA design and its applications in gene silencing.
Explore delivery methods for siRNA in cellular and animal models.
Analyze case studies of RNAi in therapeutic development.
Develop skills in interpreting RNAi data and troubleshooting experiments.

Workshop Structure

Day 1: Introduction to RNA Interference (RNAi)

Introduction to RNA Interference:

What is RNAi?
Historical background and discovery of RNAi (Fire and Mello’s work)
Overview of gene silencing pathways
Role of small RNAs in gene regulation

Mechanism of RNAi:

Step-by-step mechanism (Dicer, RISC complex)
Difference between siRNA and miRNA pathways
Key proteins involved (Argonaute, Dicer)

Biological Functions of RNAi:

Post-transcriptional gene silencing (PTGS)
Role in defense against viruses and transposons
Applications in gene expression regulation

Applications of RNAi in Research:

Functional genomics and gene knockdown
Use in model organisms (C. elegans, Drosophila, etc.)
Discussion on therapeutics and drug development

Day 2: siRNA Techniques: Design and Delivery

siRNA Basics:

What is siRNA? Structure and function
Differences between siRNA and miRNA
siRNA-mediated gene silencing

Designing siRNAs:

Key criteria for designing siRNAs
Tools and software for siRNA design (e.g., Invitrogen BLOCK-iT, siDirect)
Avoiding off-target effects
Selection of target sequences, sense and antisense strand considerations

siRNA Delivery Methods:

Lipid-based delivery systems (Lipofectamine)
Electroporation and viral vectors (AAV, lentivirus)
Nanoparticles for siRNA delivery
Challenges in siRNA delivery: Stability, off-target effects, cellular uptake

siRNA Knockdown Assays:

Confirming siRNA efficiency
Techniques: RT-qPCR, Western blotting, and immunofluorescence

Day 3: Applications of RNAi and siRNA in Research and Therapeutics

RNAi and Disease Research:

Role in cancer, neurodegenerative diseases, and viral infections
Case studies: siRNA therapies in clinical trials (Patisiran for ATTR amyloidosis)

Therapeutic Applications of siRNA:

RNAi-based drugs: Development and FDA approval (Onpattro, Givlaari)
Challenges in therapeutic siRNA delivery and stability
Ongoing clinical trials using RNAi and siRNA

Gene Therapy and siRNA:

Role of siRNA in gene therapy and personalized medicine
Ethical considerations and regulatory frameworks

Future Directions in RNAi and siRNA Research:

Next-generation RNAi technologies
CRISPR vs RNAi in gene editing

Participant’s Eligibility

Undergraduate or graduate degree in Biotechnology, Molecular Biology, Genetics, Biochemistry, or related fields.
Professionals working in genetic research, biomedical fields, or pharmaceutical industries.
Individuals interested in gene therapy, drug development, and molecular diagnostics.

Important Dates

Registration Ends

2024-12-11
Indian Standard Timing 2:00 PM

Workshop Dates

2024-12-11 to 2024-12-13
Indian Standard Timing 3:00 PM

Workshop Outcomes

Proficiency in RNAi mechanisms and siRNA design.
Practical understanding of RNAi applications in biomedical research.
Knowledge of RNAi-based therapeutic developments.
Ability to perform gene silencing experiments.
Enhanced skills in molecular diagnostics using RNA technologies.

Mentor Profile

Name: Dr. Darshan Panda
Designation: Scientist
Affiliation: Crop Physiology & Biochemistry, ICAR-NRRI

Dr. Darshan Panda is a Scientist in the Department of Crop Physiology & Biochemistry at the ICAR-National Rice Research Institute, Bidyadharpur, Cuttack. He received his Ph.D. Degree in Biotechnology from Ravenshaw University in 2022. He is working currently as a Scientist at ICAR-NRRI, Cuttack. He has published his research findings through the publication of over 50 articles in esteemed academic international journals. He has 10 years of experience in the fields of plant physiology, molecular biology and biotechnology. His area of expertise includes crop photobiology, physiology, biochemistry, molecular biology, tissue culture and biotechnology. He is the author of two textbooks in biotechnology and microbiology.

Fee Structure

Student

INR. 1399
USD. 50

Ph.D. Scholar / Researcher

INR. 1699
USD. 55

Academician / Faculty

INR. 2199
USD. 60

Student

INR. 2699
USD. 85

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