CRISPR for Cancer Genomics: A Hands-On Workshop

Edit the Genome, Decode the Cure: CRISPR Meets Cancer Genomics

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MODE
Virtual / Online

TYPE
Mentor Based

LEVEL
Moderate

DURATION
3 Days (1.5 Hours per day )

START DATE
25 -July -2025

TIME
3:00 PM IST

About

Cancer genomics and CRISPR-Cas9 technologies are reshaping modern biomedical research. This intensive 3-day workshop provides a unique platform to understand the intersection of these two revolutionary domains. Participants will explore the principles of target gene identification using TCGA/COSMIC databases, design optimized gRNAs with tools like CRISPOR and CHOPCHOP, and address off-target concerns crucial for precision genome editing.

The workshop combines theoretical modules with lab-simulated hands-on sessions, covering delivery strategies using viral and non-viral vectors, functional genomic screening with MAGeCK and PinAPL-Py, and post-screening analysis using CRISPResso and IGV. Ethical considerations and data integration practices are also discussed, preparing participants for translational cancer genomics research or industry-oriented roles.

Aim

This hands-on workshop aims to equip participants with in-depth knowledge and practical skills to harness CRISPR-Cas9 for cancer research. Through guided tutorials, real-data walkthroughs, and bioinformatics tools, participants will explore gene editing for functional genomics, target validation, and personalized oncology applications.

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Workshop Objectives

Understand gene selection from cancer genome databases (TCGA/COSMIC)
Design gRNAs using leading online platforms with off-target validation
Learn about CRISPR delivery strategies for mammalian systems
Analyze CRISPR screening data using bioinformatics tools
Integrate results for biological interpretation and ethical reporting

Workshop Structure

Day 1:Target Identification and gRNA Design

Target gene selection (TCGA/COSMIC)
Off-target prediction and design (CRISPOR, CHOPCHOP)
Hands-on: Guide RNA design

Day 2: Delivery Systems and Functional Screening

Viral/non-viral delivery (LentiGuide, AAV)
Functional screening setup (MAGeCK, PinAPL-Py)
Hands-on: Troubleshooting delivery

Day 3: Data Analysis and Validation

Data analysis (MAGeCK, CRISPResso)
Data integration and ethics (VMD, Integrative Genomics Viewer)
Hands-on: Data analysis and validation

Intended For

Undergraduate/postgraduate degree in Biotechnology, Genetics, Biomedical Engineering, Bioinformatics, or related life sciences.
Researchers and professionals working in genomics, oncology, and molecular biology domains.
Individuals interested in CRISPR, cancer research, or translational genomics.

Important Dates

Registration Ends

2025-07-25
Indian Standard Timing 2:00 PM

Workshop Dates

2025-07-25 to 2025-07-27
Indian Standard Timing 3:00 PM

Workshop Outcomes

Ability to identify and validate target genes in cancer datasets
Skills to design and test effective gRNAs
Understanding of CRISPR delivery tools and troubleshooting
Proficiency in data analysis using MAGeCK, CRISPResso, IGV
Familiarity with ethical and integrative aspects of cancer gene editing

Mentor Profile

Dr. Darshan Panda

Scientist

ICAR-NRRI

Student Fee

INR. 1399
USD. 50

Ph.D. Scholar / Researcher Fee

INR. 1699
USD. 55

Academician / Faculty Fee

INR. 2199
USD. 60

Industry Professional Fee

INR. 2699
USD. 85

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