A Hands-On Guide Using CRISPRdirect and CHOPCHOP
“Revolutionize Genetic Research with CRISPRdirect and CHOPCHOP”
Virtual (Google Meet)
Mentor Based
Moderate
3 Days (1.5 hours per day)
24 – Oct – 24
3:00 PM IST
About
CRISPR technology has revolutionized the field of genetic research, making genome editing more accessible and precise. This 3-day workshop is designed to offer an in-depth, hands-on guide to using CRISPRdirect and CHOPCHOP, two powerful tools for designing CRISPR experiments. Participants will learn how to effectively utilize these tools to create guide RNA (gRNA) for targeting specific genes, perform on-target and off-target analysis, and interpret results for different research applications.
The workshop will include real-time demonstrations, case studies, and interactive sessions to ensure participants gain the practical skills required for successful implementation of CRISPR technology in various biological systems. By the end of the workshop, attendees will be equipped to independently conduct CRISPR experiments and troubleshoot common issues associated with gRNA design and target specificity.
Aim
This workshop aims to equip researchers with the essential skills to design CRISPR experiments effectively using the popular online tools CRISPRdirect and CHOPCHOP.
Workshop Objectives
- To introduce the fundamentals of CRISPR technology.
- To guide through the use of CRISPRdirect and CHOPCHOP for designing guide RNAs.
- To provide practical knowledge on designing CRISPR experiments using these tools and interpreting the results for research applications.
Workshop Structure
Day 1: Introduction to CRISPR and CRISPR Design Tools
- CRISPR Basics: Overview of CRISPR Cas9, applications, and types of CRISPR systems.
- CRISPR Design Tools: Introduction to CRISPRdirect and CHOPCHOP
Day 2: Hands-On Training with CRISPRdirect
- CRISPRdirect Tutorial: Input target gene, select parameters, analyze results.
- Hands-On Exercise: Design sgRNAs for a gene, evaluate efficiency and specificity.
Day 3: Hands-On Training with CHOPCHOP
- CHOPCHOP Tutorial: Input target gene, select parameters, analyze results.
- Hands-On Exercise: Design sgRNAs for a different gene, compare with CRISPRdirect.
Participant’s Eligibility
- Undergraduate degree in Biotechnology, Molecular Biology, or related fields.
- Research professionals in life sciences or agricultural biotechnology.
- Students and professionals with a keen interest in genome editing and molecular genetics.
Important Dates
Registration Ends
2024-10-24
Indian Standard Timing 2:00 PM
Workshop Dates
2024-10-24 to 2024-10-26
Indian Standard Timing 3:00 PM
Workshop Outcomes
- Ability to design gRNA using CRISPRdirect and CHOPCHOP for targeted gene editing.
- Skills in interpreting and analyzing off-target and on-target effects of CRISPR experiments.
- Proficiency in applying CRISPR for plant, animal, and human gene modification projects.
- Capability to troubleshoot common issues in CRISPR-based genome editing.
- Knowledge of CRISPR applications in therapeutic, agricultural, and research settings.
Mentor Profile
Designation: Bioinformatician
Affiliation: Professional Researcher
Dr. Noopur Singh is a freelance researcher in the field of Bioinformatics. She received her Ph.D. Degree in Bioinformatics from Faculty of Engineering and Technology, Dr. A.P.J. Abdul Kalam Technical University, Lucknow, Uttar Pradesh in 2023. Prior to that, she worked as a JRF at the DBT-funded bioinformatics facility at Sri Venkateswara College, University of Delhi. Subsequently, she held the position of Assistant Professor in the Department of Biotechnology at Dr. Ambedkar Institute of Technology for Handicapped, Kanpur. Dr. Noopur has over 10 years of experience in the field of Bioinformatics. Her area of expertise includes: Computer Aided Drug Design, Molecular Modeling, Gene Editing, NGS Data Analysis, Sequence Analysis, Gene Prediction. She has authored 7 research publications.
Fee Structure
Student
INR. 1399
USD. 50
Ph.D. Scholar / Researcher
INR. 1699
USD. 55
Academician / Faculty
INR. 2199
USD. 60
Industry Professional
INR. 2699
USD. 85
List of Currencies
Key Takeaways
- Access to Live Lectures
- Access to Recorded Sessions
- e-Certificate
- Query Solving Post Workshop
Future Career Prospects
- Genome Editing Scientist: Work in research and development in academia or industry, focusing on genetic modification projects.
- Biotech Research Analyst: Analyze genetic data and contribute to the development of new biotech products.
- CRISPR Product Specialist: Serve as a technical expert in biotechnology firms that offer CRISPR-related products and services.
- Genetic Counselor: Assist in providing information related to genetic modifications and gene therapy.
- Plant Geneticist: Apply CRISPR technology to develop genetically modified crops with improved traits.
- Medical Research Scientist: Use CRISPR for therapeutic applications such as gene therapy and disease modeling.
Job Opportunities
- Research institutions, academic labs, and universities.
- Biotech and pharmaceutical companies focused on gene editing and molecular research.
- Agricultural biotechnology firms working on crop improvement.
- Healthcare companies developing gene therapies.
- Government and private research organizations.
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