Comet Assay to Study Antimutagenic Activity of Different Compounds

Decoding DNA Protection: Explore the Power of Antimutagenic Compounds

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MODE
Online/ e-LMS

TYPE
Self Paced

LEVEL
Moderate

DURATION
1 Month

About

The “Comet Assay to Study Antimutagenic Activity of Different Compounds” program is designed to provide a deep dive into the comet assay (single cell gel electrophoresis), a versatile and sensitive technique for measuring DNA damage and repair. Participants will learn the foundational principles and step-by-step methodologies of conducting comet assays, as well as how to interpret and utilize the results in practical and research settings.

Aim

This program aims to equip participants with the skills to conduct comet assays to evaluate the antimutagenic properties of various compounds. It focuses on understanding the interaction between these compounds and DNA, methods to mitigate genetic damage, and the implications for cancer research and environmental science.

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Program Objectives

Master the comet assay technique for detecting DNA damage.
Understand the principles of antimutagenic activity and its measurement.
Develop skills to analyze and interpret assay data effectively.
Explore the use of comet assay in various applied sciences including toxicology and pharmacology.
Learn about current trends and future directions in genetic damage prevention.

Program Structure

Modues 1: Introduction to Comet Assay

What is Comet Assay?

Applications of Comet Assay

The Principle of Comet Assay

Why is Comet Assay important?

Theoretical background of Comet Assay

Protocol for Performing Comet Assay
Sample preparation
Electrophoresis
Visualization and analysis of DNA damage
Q&A Session
Analysis of Results of Comet Assay
Interpretation of Comet Assay data
Quantification of DNA damage
Uses of Comet Assay
How Comet Assay is used in toxicology and genotoxicity studies
Future applications of Comet Assay
Modifications or Improvisations of Comet Assay
Various modifications and improvements to the original Comet Assay protocol
Advantages and limitations of different modifications
Q&A Session

Intended For

Undergraduate degree in Biology, Biochemistry, Environmental Science, or related fields.
Professionals in pharmaceutical, biotechnology, or environmental sectors.
Individuals with an interest in genetic research and its applications in health and safety.

Program Outcomes

Proficiency in performing comet assays
Knowledge of genetic damage mechanisms and repair
Skills in data collection and interpretation
Understanding of compound interactions with DNA
Preparation for roles in genetic and environmental health research

Fee Structure

Standard Fee: INR ~~4,998~~ USD ~~110~~

Discounted Fee: INR 2499 USD 55

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