Advanced Digital PCR (dPCR) Multiplexing: Principles, Design, and Applications
Next-Gen Diagnostics Through Multiplex dPCR: Accuracy in Every Assay
About This Course
This 3-day hands-on workshop offers an in-depth exploration of multiplex digital PCR (dPCR)—a cutting-edge technique for absolute quantification of nucleic acids. Participants will learn the principles of dPCR, design multiplex assays, troubleshoot experiments, and analyze real-world data using simulation tools. Applications span infectious disease diagnostics, cancer mutation detection, gene expression analysis, and more. Ideal for molecular biologists, diagnostic researchers, and biotech professionals seeking advanced skills in high-precision PCR technologies.
Aim
The aim of this workshop is to equip participants with a comprehensive understanding of digital PCR (dPCR) technology, with a special emphasis on multiplex assay design, optimization, and application. Through a combination of theoretical instruction, case-based learning, and hands-on simulation, the workshop intends to enable researchers, clinicians, and molecular biologists to leverage multiplex dPCR for precise, absolute quantification of nucleic acids across a range of fields including infectious disease diagnostics, oncology, genetic mutation detection, and GMO testing.
Workshop Objectives
- To introduce the fundamentals of digital PCR and its advantages over qPCR
- To explain the principles and applications of multiplex assay design
- To train participants in primer/probe design and spectral optimization
- To provide hands-on guidance on dPCR instrument operation and data analysis
- To demonstrate the application of dPCR in clinical and research settings
- To enhance skills in interpreting multiplex results and troubleshooting experiments
Workshop Structure
Day 1: Fundamentals of dPCR
- Introduction to Digital PCR vs qPCR
- Principles of droplet and chip-based dPCR
- Concept of absolute quantification
- Applications overview (oncology, virology, plant biotech)
Day 2: Multiplexing Design & Optimization
- Primer/probe design strategies
- Avoiding dimer formation and spectral overlap
- Multiplex assay optimization techniques
- Hands-on software simulation: multiplex setup
Day 3: Hands-On dPCR & Data Interpretation
- Running a multiplex dPCR experiment
- Instrument calibration and loading
- Endpoint fluorescence reading and analysis
- Data interpretation, troubleshooting common issues
- Case Study: SARS-CoV-2 and Influenza co-detection
Who Should Enrol?
- Molecular biologists, geneticists, and microbiologists
- Biotech and life science researchers working in diagnostics, oncology, virology, or agriculture
- Postgraduate and PhD students in biotechnology, bioinformatics, or related disciplines
- Clinical lab professionals and diagnostic R&D teams
- Anyone interested in mastering digital PCR and multiplex assay design
Important Dates
Registration Ends
09/30/2025
IST 2:00 PM
Workshop Dates
09/30/2025 – 10/02/2025
IST 3:00 PM
Workshop Outcomes
- Understand the principles and workflow of digital PCR and multiplexing
- Be able to design and optimize multiplex dPCR assays
- Gain hands-on experience with dPCR setup, calibration, and data analysis
- Learn to troubleshoot common issues in multiplex assays
- Apply dPCR knowledge to real-world cases in infectious disease, oncology, and GMO detection
- Receive a certificate of participation validating their practical and theoretical competence
Fee Structure
Student Fee
₹1399 | $50
Ph.D. Scholar / Researcher Fee
₹1699 | $60
Academician / Faculty Fee
₹2199 | $70
Industry Professional Fee
₹2699 | $80
What You’ll Gain
- Live & recorded sessions
- e-Certificate upon completion
- Post-workshop query support
- Hands-on learning experience
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