New Year Offer End Date: 30th April 2024
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Program

Metagenomic Analysis of AMR and HGT

Uncovering the Hidden World of Resistance: AI Meets Metagenomics

Skills you will gain:

About Program:

Antimicrobial resistance and horizontal gene transfer are critical public health challenges, as they facilitate the emergence of multidrug-resistant pathogens. Metagenomics offers a powerful way to uncover resistance mechanisms and gene exchange within complex microbial ecosystems.
This workshop provides hands-on training in metagenomic sequence analysis, gene prediction, resistome profiling, and mobile genetic element identification. Participants will learn to use tools and databases such as CARD, MEGARes, ResFinder, and ICEberg for AMR detection, and employ AI and machine learning approaches for classifying and visualizing gene transfer events.

Aim:

This workshop aims to introduce participants to advanced computational and AI-driven approaches for analyzing antimicrobial resistance (AMR) and horizontal gene transfer (HGT) using metagenomic datasets. The program will focus on data processing, functional gene annotation, and network-based prediction to understand the spread and evolution of resistance genes in microbial communities.

Program Objectives:

  • Understand AMR and HGT mechanisms and their relevance to microbial ecology and public health.
  • Learn metagenomic data processing — quality control, assembly, annotation, and classification.
  • Use computational tools (CARD, MEGARes, ResFinder) for resistome and mobilome profiling.
  • Apply AI/ML models for AMR prediction and HGT detection.
  • Develop data visualization and interpretation skills for metagenomic results.

What you will learn?

Day 1: Introduction & Data Pre-Processing

  • Overview of Metagenomics, Resistome, and HGT Mechanisms.
  • NGS data formats, Quality control using FastQC and Trimmomatic.
  • Hands-on: Metagenomic assembly using MEGAHIT / SPAdes.
  • Contig annotation using Prokka or MGnify workflows.

Day 2: ARG and MGE Detection

  • Databases — MEGARes, CARD, DeepARG, and ResFinder.
  • Hands-on: Running DeepARG for ARG detection and abundance estimation.
  • Hands-on: Identifying plasmids and transposons using PlasFlow and MobileElementFinder.
  • Integrating ARG and MGE profiles for HGT potential assessment

Day 3: Visualization & Interpretation

  • Hands-on: Visualization of ARG abundance using R (ggplot2) and Python (matplotlib/seaborn).
  • Comparative analysis of environmental vs. clinical resistomes.
  • Resistome diversity plots and HGT network maps.

Mentor Profile

Fee Plan

INR 1999 /- OR USD 50

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Intended For :

  • Undergraduate/Postgraduate degree in Microbiology, Biotechnology, Bioinformatics, Computational Biology, or related fields.
  • Professionals working in genomics, clinical microbiology, environmental microbiology, or infectious disease research.
  • Data scientists and AI/ML engineers aiming to apply computational models in biological and public health data.
  • Individuals interested in understanding microbial evolution, antibiotic resistance, and bioinformatics workflows.

Career Supporting Skills

Metagenomics Annotation MachineLearning Python CARD MEGARes DataVisualization

Program Outcomes

  • Mastery of workflows for metagenomic analysis of AMR and HGT.
  • Ability to detect, annotate, and visualize resistance genes in microbial communities.
  • Understanding of AI-driven predictive modeling for resistance and transfer events.
  • Hands-on experience with CARD, MEGARes, and related databases.
  • Competence in integrating biological insights with computational results.