New Year Offer End Date: 30th April 2024
Program

CRISPR for Cancer Genomics: A Hands-On Workshop

Edit the Genome, Decode the Cure: CRISPR Meets Cancer Genomics

Skills you will gain:

About Program:

Cancer genomics and CRISPR-Cas9 technologies are reshaping modern biomedical research. This intensive 3-day workshop provides a unique platform to understand the intersection of these two revolutionary domains. Participants will explore the principles of target gene identification using TCGA/COSMIC databases, design optimized gRNAs with tools like CRISPOR and CHOPCHOP, and address off-target concerns crucial for precision genome editing.

The workshop combines theoretical modules with lab-simulated hands-on sessions, covering delivery strategies using viral and non-viral vectors, functional genomic screening with MAGeCK and PinAPL-Py, and post-screening analysis using CRISPResso and IGV. Ethical considerations and data integration practices are also discussed, preparing participants for translational cancer genomics research or industry-oriented roles.

Aim: This hands-on workshop aims to equip participants with in-depth knowledge and practical skills to harness CRISPR-Cas9 for cancer research. Through guided tutorials, real-data walkthroughs, and bioinformatics tools, participants will explore gene editing for functional genomics, target validation, and personalized oncology applications.

Program Objectives:

  • Understand gene selection from cancer genome databases (TCGA/COSMIC)

  • Design gRNAs using leading online platforms with off-target validation

  • Learn about CRISPR delivery strategies for mammalian systems

  • Analyze CRISPR screening data using bioinformatics tools

  • Integrate results for biological interpretation and ethical reporting

What you will learn?

Day 1:Target Identification and gRNA Design

  • Target gene selection (TCGA/COSMIC)
  • Off-target prediction and design (CRISPOR, CHOPCHOP)
  • Hands-on: Guide RNA design

Day 2: Delivery Systems and Functional Screening

  • Viral/non-viral delivery (LentiGuide, AAV)
  • Functional screening setup (MAGeCK, PinAPL-Py)
  • Hands-on: Troubleshooting delivery

Day 3: Data Analysis and Validation

  • Data analysis (MAGeCK, CRISPResso)
  • Data integration and ethics (VMD, Integrative Genomics Viewer)
  • Hands-on: Data analysis and validation

Mentor Profile

Scientist ICAR-NRRI
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Fee Plan

INR 1999 /- OR USD 50

Get an e-Certificate of Participation!

Intended For :

  • Undergraduate/postgraduate degree in Biotechnology, Genetics, Biomedical Engineering, Bioinformatics, or related life sciences.
  • Researchers and professionals working in genomics, oncology, and molecular biology domains.
  • Individuals interested in CRISPR, cancer research, or translational genomics.

Career Supporting Skills

gRNA-design Bioinformatics Data-analysis CRISPR Genomics

Program Outcomes

  • Ability to identify and validate target genes in cancer datasets

  • Skills to design and test effective gRNAs

  • Understanding of CRISPR delivery tools and troubleshooting

  • Proficiency in data analysis using MAGeCK, CRISPResso, IGV

  • Familiarity with ethical and integrative aspects of cancer gene editing

FREEDOM TO LEARN 10% OFF All Courses & Workshops Use Code: NANOINDIA10 ⏳ Offer Ends In: Loading... Learn Today. Lead Tomorrow. Explore Programs →
FREEDOM TO LEARN 10% OFF All Courses & Workshops Use Code: NANOINDIA10 ⏳ Offer Ends In: Loading... Learn Today. Lead Tomorrow. Explore Programs →