New Year Offer End Date: 30th April 2024
Program
CRISPR-Cas Genome Editing: Workflow, Tools and Techniques
Unlocking Precision Gene Editing: CRISPR-Cas Workflow, Tools, and Techniques
About Program:
CRISPR-Cas gene editing technology is a powerful tool that has revolutionized the field of biology. It allows researchers to make precise and efficient changes to the genome of any organism. This has led to a rapid increase in our understanding of gene function and disease mechanisms, and it has also opened up new possibilities for gene therapy and other therapeutic applications.
In this program, participants will learn about the basics of CRISPR-Cas gene editing, its advanced applications and ethical considerations. The program will include lectures, hands-on demonstrations, and discussions with experts in the field.
Aim: The program aims to provide participants with a comprehensive overview of CRISPR-Cas gene editing technology and its applications. The program will cover the basics of CRISPR-Cas, including how it works, different types of CRISPR-Cas systems, and its potential impact on various fields, such as basic research, translational research, and gene therapy.
Program Objectives:
- Educational Foundation: To provide participants with a solid foundation in the principles and mechanisms of CRISPR-Cas genome editing, including the roles of guide RNAs, Cas proteins, and DNA repair pathways.
- Hands-On Training: To offer practical, hands-on experience in CRISPR-Cas genome editing techniques, including guide RNA design, cell or organism manipulation, and molecular analysis of editing outcomes.
- Application Across Disciplines: To demonstrate the diverse applications of CRISPR-Cas genome editing in fields such as basic research, biotechnology, agriculture, medicine, and beyond.
- Critical Thinking and Problem-Solving: To develop participants’ critical thinking skills to evaluate experimental results, troubleshoot technical challenges, and design effective strategies for genome editing experiments.
- Ethical and Societal Considerations: To introduce participants to the ethical, legal, and societal implications of CRISPR-Cas genome editing, including considerations related to safety, equity, consent, and regulatory oversight.
- Communication and Collaboration: To foster effective communication and collaboration skills through written and oral presentations, collaborative projects, and discussions with peers and experts in the field.
- Career Development: To provide resources and guidance to help participants explore career paths related to CRISPR-Cas genome editing, including opportunities for internships, research projects, and networking with professionals in academia, industry, and other sectors.
- Research Advancement: To contribute to the advancement of research in CRISPR-Cas genome editing by fostering a community of scholars, researchers, and practitioners who can collaborate and share knowledge and expertise.
What you will learn?
Day 1: Introduction and applications of CRISPR-Cas
- What is CRISPR-Cas?
- How does CRISPR-Cas work?
- Different types of CRISPR-Cas systems
- CRISPR in Basic Research
- CRISPR in Translational Research
- CRISPR in Gene therapy
Day 2: CRISPR-Cas gene editing workflows and challenges and troubleshooting
- Designing a CRISPR-Cas experiment
- Assembling CRISPR-Cas components
- Transfecting or transducing cells with CRISPR-Cas components
- Validating gene editing results
- Off-target effects
- Low efficiency
- Delivery challenges
Day 3: Advanced CRISPR-Cas gene editing techniques, ethics and regulation
- CRISPR-Cas gene activation and repression
- CRISPR-Cas gene editing in primary cells and tissues
- CRISPR-Cas genome engineering for animal models
- Responsible use of CRISPR-Cas
- Intellectual property considerations
- Regulatory landscape for CRISPR-Cas gene editing
Fee Plan
INR 1999 /- OR USD 50
Intended For :
- Educational Background: Typically, applicants should have a background in biology, genetics, molecular biology, biochemistry, or a related field. This could include undergraduate or graduate degrees.
- Experience: Some programs might require applicants to have prior experience or coursework in molecular biology techniques, genetic engineering, or related laboratory skills.
- Prerequisites: Depending on the level of the program, there may be specific prerequisites such as coursework in genetics, biochemistry, or molecular biology.
- Professional Status: Programs might be open to undergraduate students, graduate students, postdoctoral researchers, or professionals in related fields such as biotechnology or pharmaceuticals.
- Language Proficiency: If the program is conducted in a language other than the applicant’s native language, proficiency in that language might be required.
- Application Materials: Applicants might need to submit materials such as transcripts, letters of recommendation, a statement of purpose, and/or a resume or CV.
- Visa Requirements: For international applicants, there may be visa requirements to consider.
- Funding: Some programs may offer scholarships or require applicants to demonstrate funding for tuition and living expenses.
Career Supporting Skills
Advanced Gene Editing Techniques Advanced CRISPR-Cas Techniques CRISPR-Cas Understanding Cell Transfection and Validation Precision in Gene Editing Precision Medicine
Program Outcomes
- Understanding of CRISPR-Cas Technology: Participants will gain a comprehensive understanding of the principles and mechanisms underlying CRISPR-Cas genome editing, including how CRISPR systems function, how guide RNAs target specific DNA sequences, and how Cas proteins modify genomic DNA.
- Proficiency in Genome Editing Techniques: Participants will develop practical skills in designing and implementing CRISPR-Cas genome editing experiments, including designing guide RNAs, delivering CRISPR components into cells or organisms, and analyzing editing outcomes using molecular biology techniques.
- Knowledge of Applications and Limitations: Participants will learn about the diverse applications of CRISPR-Cas genome editing in areas such as basic research, biotechnology, agriculture, and medicine. They will also gain an understanding of the limitations and challenges associated with CRISPR technology, such as off-target effects and delivery methods.
- Critical Thinking and Problem-Solving Skills: Through hands-on laboratory work, case studies, and discussions, participants will develop critical thinking skills to evaluate experimental results, troubleshoot technical challenges, and design strategies to optimize CRISPR experiments.
- Ethical and Regulatory Awareness: Participants will be introduced to the ethical, legal, and societal implications of CRISPR-Cas genome editing, including considerations related to safety, equity, and consent. They will also gain an understanding of the regulatory frameworks governing the use of genome editing technologies in different contexts.
- Communication and Collaboration Skills: Participants will have opportunities to communicate their research findings, ideas, and ethical perspectives effectively through written reports, oral presentations, and collaborative projects. They will also develop teamwork and interpersonal skills by working with peers from diverse backgrounds.
- Career Preparation and Networking: The program may provide resources and guidance to help participants explore career paths related to CRISPR-Cas genome editing, including opportunities for internships, research projects, or networking with professionals in academia, industry, and other sectors.
