Attribute

Detail

Format

Recorded Lectures (Self-Paced)

Level

Intermediate

Duration

3 Days

Certification

e-Certification + e-Marksheet

Tools

Galaxy Platform, Hisat2, FeatureCounts, DESeq2, FastQC, MultiQC

About the Ngs Data Analysis Course

Next-Generation Sequencing (NGS) has revolutionized the field of genomics by enabling the rapid sequencing of entire genomes and transcriptomes. This course offers an in-depth exploration of NGS data analysis using the Galaxy platform, a powerful and user-friendly web-based tool. Participants will learn to navigate the Galaxy interface, perform quality control, align sequences, and interpret results.

The course is designed to provide both theoretical knowledge and hands-on experience, ensuring that participants can confidently apply these techniques in real-world research settings. Galaxy is an open-source platform that simplifies complex bioinformatics workflows, making it accessible to researchers without extensive programming skills. Throughout the course, participants will engage in practical exercises and projects that simulate actual research scenarios. By the end of the course, they will be equipped with the skills necessary to process and analyze NGS data, contributing to advancements in genomics, personalized medicine, and other cutting-edge fields.

Program Highlights

• Comprehensive coverage of Next from fundamentals to advanced applications

• Hands-on projects and real-world case studies in Bioinformatics

• Expert-curated curriculum aligned with current industry standards

• Access to recorded lectures and e-LMS platform for flexible, self-paced learning

• e-Certification and e-Marksheet upon successful completion

• Dedicated mentor support and interactive doubt-clearing sessions

• Practical experience with tools: Galaxy Platform, Hisat2, FeatureCounts, DESeq2

• Career-oriented training for academic and professional growth in Bioinformatics

Course Curriculum

Module 1: Foundations of NGS and Galaxy Platform

Master the fundamental principles and concepts of Next-Generation Sequencing technology
Navigate the Galaxy interface and configure user accounts for bioinformatics workflows
Execute quality control assessments and preprocessing steps for raw NGS data

Module 2: Genome Assembly and Annotation

Utilize Galaxy tools for de novo and reference-guided genome assembly
Apply automated annotation pipelines to predict gene structures and functional elements
Validate assembly quality and assess completeness using benchmark metrics

Module 3: Variant Identification and Analysis

Perform read mapping and alignment against reference genomes using industry-standard algorithms
Identify single nucleotide polymorphisms (SNPs) and structural variants from NGS data
Filter, annotate, and prioritize variants for clinical and research applications

Module 4: RNA-Seq Analysis and Transcriptome Assembly

Process raw RNA-Seq reads using Hisat2 for accurate splice-aware alignment
Quantify gene and transcript abundance with FeatureCounts and related tools
Assemble transcriptomes and discover novel isoforms from sequencing data

Module 5: Differential Gene Expression with DESeq2

Design robust differential expression experiments with proper biological replicates
Execute DESeq2 workflows within Galaxy to identify significantly regulated genes
Visualize expression patterns through volcano plots, heatmaps, and PCA analyses

Module 6: Pathway Analysis and Functional Interpretation

Perform Gene Ontology (GO) enrichment and pathway over-representation analysis
Map differentially expressed genes to biological pathways and disease associations
Interpret multi-omics results to generate actionable biological hypotheses

Module 7: Pipeline Construction and Workflow Optimization

Construct reproducible, automated analysis pipelines using Galaxy's workflow editor
Implement best practices and optimization strategies for efficient NGS data analysis
Customize parameters and troubleshoot common bioinformatics challenges

Tools, Techniques, or Platforms Covered

Galaxy Platform
Hisat2
FeatureCounts
DESeq2
FastQC
MultiQC

Real-World Applications

Apply Next skills directly to academic research, thesis work, and publications
Build a professional portfolio showcasing practical Bioinformatics competencies
Solve industry-relevant problems using Next methodologies and tools
Contribute to open-source projects and collaborative research in Bioinformatics
Prepare for competitive examinations, interviews, and professional certifications in Bioinformatics

Who Should Attend & Prerequisites

Industry-recognized e-Certification + e-Marksheet from NSTC
Hands-on training with practical projects and industrial datasets
Dedicated expert mentorship and doubt resolution

Prerequisites:

Frequently Asked Questions

1. What is the format of this Next-Generation Sequencing Data Analysis using Galaxy Platform course?

This is an Recorded Lectures (Self-Paced) course delivered via our e-LMS platform. You will have access to pre-recorded video lectures, reading materials, assignments, quizzes, and hands-on projects that you can complete at your own pace.

2. Will I receive a certificate after completing this course?

Yes! Upon successful completion of all modules, assignments, and assessments, you will receive an e-Certification along with an e-Marksheet from NanoSchool (NSTC) that you can showcase on your CV and LinkedIn profile.

3. What are the prerequisites for this course?

Learners should have a foundational understanding of Bioinformatics concepts. Familiarity with basic tools and programming is recommended.

4. How long will I have access to the course materials?

You will have access to all course materials for the duration of 3 Days. The self-paced format allows you to learn according to your own schedule through our online learning management system.

5. Is mentor support available during the course?

Yes, dedicated mentor support is available throughout the course. You can reach out for doubt-clearing sessions, project guidance, and career advice related to Bioinformatics. Our mentors are industry experts and experienced professionals.

Enroll in Next-Generation Sequencing Data Analysis using Galaxy Platform today and take the next step in your professional journey. With expert-curated content, practical projects, and industry-recognized certification, this course is your gateway to mastering Bioinformatics skills that matter.

Brand	NSTC
Format	Recorded Lectures
Duration	3 Days
Level	Intermediate
Domain	Bioinformatics
Hands-On	Yes – Practical projects with industrial datasets
Tools Used	Galaxy Platform, Hisat2, FeatureCounts, DESeq2, FastQC, MultiQC

Achieve Excellence & Enter the Hall of Fame!

Elevate your research to the next level! Get your groundbreaking work considered for publication in prestigious Open Access Journal (worth USD 1,000) and Opportunity to join esteemed Centre of Excellence. Network with industry leaders, access ongoing learning opportunities, and potentially earn a place in our coveted

Hall of Fame.

Achieve excellence and solidify your reputation among the elite!