Attribute

Detail

Format

Recorded Lectures (Self-Paced)

Level

Intermediate

Duration

3 Days (1.5 Hours per day)

Certification

e-Certification + e-Marksheet

Tools

Flow cytometer, FlowJo, Cytobank, FCS files, t-SNE, UMAP

About the Flow Cytometry Course

Flow cytometry is a cornerstone technology in immunology, cancer biology, hematology, and cell therapy, enabling rapid multiparametric analysis of thousands of cells per second.

This 3‑day intensive blends experimental technique with analytical know‑how – from sample handling and panel design to manual gating and an introduction to high‑dimensional single‑cell analytics (t‑SNE/UMAP). Learn by doing with real FCS files, hands‑on instrument setup, and guided data‑visualisation.

Program Highlights

• Comprehensive coverage of Flow Cytometry from fundamentals to advanced applications

• Hands-on projects and real-world case studies in biotechnology

• Expert-curated curriculum aligned with current industry standards

• Access to recorded lectures and e-LMS platform for flexible, self-paced learning

• e-Certification and e-Marksheet upon successful completion

• Dedicated mentor support and interactive doubt-clearing sessions

• Practical experience with tools: Flow cytometer, FlowJo, Cytobank, FCS files

• Career-oriented training for academic and professional growth in biotechnology

Course Curriculum

Module 1: Day 1 – Basics & Sample Preparation

Understand flow cytometry principles, FSC/SSC and fluorescence channels
Perform cell washing, counting, and concentration adjustment
Execute staining protocols with fluorochrome‑tagged antibodies

Module 2: Day 2 – Instrument Setup, Acquisition & Gating

Configure voltages, fluidics and select optimal parameters
Run controls and stained samples on the cytometer
Apply live gating, compensation and export gated data

Module 3: Day 3 – Panel Design, Data Analysis & Plots

Design simple panels, match markers to fluorochromes and minimize spillover
Calculate population percentages, MFI and compare experimental conditions
Generate publication‑ready histograms, dot plots and explore t‑SNE/UMAP concepts

Tools, Techniques, or Platforms Covered

Flow cytometer
FlowJo
Cytobank
FCS files
t-SNE
UMAP

Real-World Applications

Apply Flow Cytometry skills directly to academic research, thesis work, and publications
Build a professional portfolio showcasing practical biotechnology competencies
Solve industry-relevant problems using Flow Cytometry methodologies and tools
Contribute to open-source projects and collaborative research in biotechnology
Prepare for competitive examinations, interviews, and professional certifications in biotechnology

Who Should Attend & Prerequisites

Industry‑recognized e‑Certification + e‑Marksheet from NSTC
Hands‑on training with practical projects and real datasets
Dedicated expert mentorship and doubt‑resolution sessions

Prerequisites:

Frequently Asked Questions

1. What is the format of this Flow Cytometry & Single-Cell Analysis: From Sample to Data course?

This is an Recorded Lectures (Self-Paced) course delivered via our e-LMS platform. You will have access to pre-recorded video lectures, reading materials, assignments, quizzes, and hands-on projects that you can complete at your own pace.

2. Will I receive a certificate after completing this course?

Yes! Upon successful completion of all modules, assignments, and assessments, you will receive an e-Certification along with an e-Marksheet from NanoSchool (NSTC) that you can showcase on your CV and LinkedIn profile.

3. What are the prerequisites for this course?

Learners should have a foundational understanding of biotechnology concepts. Familiarity with basic tools and programming is recommended.

4. How long will I have access to the course materials?

You will have access to all course materials for the duration of 3 Days (1.5 Hours per day). The self-paced format allows you to learn according to your own schedule through our online learning management system.

5. Is mentor support available during the course?

Yes, dedicated mentor support is available throughout the course. You can reach out for doubt-clearing sessions, project guidance, and career advice related to biotechnology. Our mentors are industry experts and experienced professionals.

Enroll in Flow Cytometry & Single-Cell Analysis: From Sample to Data today and take the next step in your professional journey. With expert-curated content, practical projects, and industry-recognized certification, this course is your gateway to mastering biotechnology skills that matter.

Brand	NSTC
Format	Recorded Lectures
Duration	3 Days (1.5 Hours per day)
Level	Intermediate
Domain	biotechnology
Hands-On	Yes – Practical projects with industrial datasets
Tools Used	Flow cytometer, FlowJo, Cytobank, FCS files, t-SNE, UMAP

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