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CRISPR-Cas Genome Editing: Workflow, Tools and Techniques
USD $59.00 USD $249.00Price range: USD $59.00 through USD $249.00
Aim: The workshop aims to provide participants with a comprehensive overview of CRISPR-Cas gene editing technology and its applications. The workshop will cover the basics of CRISPR-Cas, including how it works, different types of CRISPR-Cas systems, and its potential impact on various fields, such as basic research, translational research, and gene therapy.
Aim
CRISPR-Cas Genome Editing: Workflow, Tools and Techniques trains participants to understand and plan end-to-end CRISPR genome editing experiments—from target selection and guide design to delivery, screening, validation, and responsible reporting. You’ll learn practical workflows, tool-driven decision-making, experimental controls, and off-target awareness—so you can design credible editing strategies for research and translational pipelines.
Program Objectives
- Understand CRISPR Systems: Cas9/Cas12 basics, PAM rules, and repair pathways (NHEJ vs HDR) at a practical level.
- Plan Editing Workflows: Knockout, knock-in, base editing, and CRISPRi/a (overview + use-case mapping).
- Design Guides Correctly: gRNA design logic, on-target scoring, off-target risk reduction, and controls.
- Choose Delivery Methods: Plasmid, RNP, viral delivery concepts; cell-type constraints and trade-offs.
- Screen & Validate Edits: PCR, sequencing, genotyping approaches, and functional validation strategy.
- Use Tools Efficiently: Introduce key software/web tools for design, QC, and analysis.
- Work Safely & Ethically: Biosafety awareness, documentation, and responsible communication.
- Hands-on Application: Build a complete CRISPR experimental design package as a capstone.
Program Structure
Module 1: CRISPR-Cas Basics (What You Need for Real Work)
- CRISPR overview: guide RNA + nuclease + target DNA concept.
- Cas systems: Cas9 vs Cas12; PAM recognition and cut patterns (high-level).
- Repair pathways: NHEJ vs HDR; why outcomes vary by cell type and timing.
- Editing readouts: indels, frameshifts, precise edits, mosaicism (conceptual).
Module 2: Defining the Editing Goal & Experimental Strategy
- Choosing approach: knockout vs knock-in vs base editing vs CRISPRi/a.
- Target selection: gene regions, isoforms, essentiality, and experiment constraints.
- Controls planning: non-targeting controls, positive controls, mock delivery.
- Reference sequences and annotation hygiene (transcripts, exons, variants).
Module 3: gRNA Design Workflow (Scoring, Filters, Off-Targets)
- Guide design principles: PAM availability, GC%, seed region, secondary structures (intro).
- On-target scoring and common heuristics for robust guide selection.
- Off-target concepts: mismatch tolerance, genome context, repetitive regions.
- Practical use of design tools (overview): choosing guides and documenting decisions.
Module 4: Donor Design & HDR Planning (When You Need Precision)
- When HDR is necessary: tags, point mutations, insertions (conceptual).
- Donor options: ssODN vs plasmid donors; homology arms basics.
- Protecting edits: silent PAM disruption, re-cut prevention (intro).
- Enhancing HDR (conceptual): timing, cell cycle considerations, trade-offs.
Module 5: Delivery Methods & Practical Constraints
- Delivery options: plasmids, mRNA, RNP complexes—pros/cons.
- Viral delivery overview: AAV/lenti concepts and when they’re used (high-level).
- Cell type constraints: primary cells vs cell lines; viability and efficiency trade-offs.
- Optimization mindset: dose, toxicity, timing, and controls.
Module 6: Screening & Genotyping (From Cells to Answers)
- Early screening: PCR assays, restriction digest concepts, T7E1-style logic (intro).
- Sanger vs NGS: what each can confirm and what they can’t.
- Clonal isolation vs bulk editing: when each makes sense.
- Quantifying editing outcomes: indel percentage, allele distribution (conceptual).
Module 7: Off-Target Assessment & Functional Validation
- Off-target validation strategies: predicted sites, sequencing checks (high-level).
- Functional validation: phenotype, protein expression, pathway assays (planning level).
- Common pitfalls: false positives, clonal artifacts, compensation effects.
- Documentation: reporting assumptions, limitations, and reproducibility notes.
Module 8: Workflow Automation, Data Handling & Responsible Reporting
- Organizing CRISPR projects: sample naming, metadata, storage, versioning.
- Tools overview: guide design, primer design, alignment/variant interpretation (workflow view).
- Writing Methods that replicate: parameters, sequences, and decision log requirements.
- Ethics and scope: research vs clinical claims; safety and compliance awareness.
Final Project
- Select a target gene and an editing goal (KO, KI, base edit, or CRISPRi/a).
- Prepare a complete workflow: target rationale, guide selection, delivery plan, controls, and timelines.
- Design a screening + validation plan (genotyping + functional readouts).
- Deliverables: CRISPR design dossier + guide table + workflow diagram + validation checklist.
Participant Eligibility
- UG/PG students and researchers in Biotechnology, Genetics, Molecular Biology, Bioinformatics
- PhD scholars/postdocs planning gene editing experiments
- Industry professionals in biotech R&D and molecular platforms
- Anyone with basic molecular biology knowledge aiming to learn CRISPR workflows
Program Outcomes
- Workflow Competence: Ability to plan end-to-end CRISPR experiments with clear controls and documentation.
- Guide Design Confidence: Ability to choose guides and justify selection with on/off-target reasoning.
- Delivery & Screening Readiness: Ability to select delivery methods and build a realistic screening plan.
- Validation Mindset: Ability to plan genotyping and functional validation responsibly.
- Portfolio Deliverable: A complete CRISPR experimental design dossier you can showcase.
Program Deliverables
- Access to e-LMS: Full access to lectures, worksheets, and tool links.
- CRISPR Toolkit Pack: Target selection sheet, gRNA scoring table, HDR donor template, controls checklist.
- Validation Templates: Genotyping workflow template, sequencing checklist, results reporting outline.
- Case Studies: Knockout/knock-in/base-edit examples and design trade-offs.
- Hands-on Project Support: Guided feedback on capstone design and troubleshooting logic.
- Final Assessment: Certification after assignments + capstone submission.
- e-Certification and e-Marksheet: Digital credentials provided upon successful completion.
Future Career Prospects
- Genome Editing Research Associate
- Molecular Biology / Cell Engineering Associate
- CRISPR Platform Support Specialist (R&D)
- Biotech R&D Associate (Gene Therapy / Synthetic Biology)
- Bioinformatics Associate (CRISPR Design & Validation Support)
Job Opportunities
- Biotech & Pharma R&D: Gene editing workflows, cell engineering, assay development support.
- Gene Therapy & Cell Therapy Companies: Editing design, validation, and process development support.
- Academic & Research Institutes: CRISPR research projects, functional genomics labs, core facilities.
- CROs & Core Facilities: Editing services, genotyping pipelines, and documentation/reporting.
- Synthetic Biology Startups: Rapid design-build-test cycles, automation, and validation workflows.
| Category | E-LMS, E-LMS+Video, E-LMS+Video+Live Lectures |
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