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Development of C4 Plants Using rDNA Technology
Original price was: INR ₹5,499.00.INR ₹2,999.00Current price is: INR ₹2,999.00.
Development of C4 Plants Using rDNA Technology is an intermediate-level, 1-month online course by NSTC. Explore the principles of C4 photosynthesis, recombinant DNA technology, gene transfer, and crop improvement through practical projects, real-world case studies, and expert mentorship. Earn an e-Certificate and e-Marksheet upon successful completion.
Internship Details
About the Internship
The Development of C4 Plants Using rDNA Technology Internship introduces learners to C3 and C4 photosynthesis, recombinant DNA technology, plant genetic engineering, gene regulation, and crop improvement.
Participants will understand how C4-related genes and pathways can be introduced into C3 crops such as rice and wheat to improve photosynthetic efficiency, water-use efficiency, productivity, and climate resilience.
The internship includes guided activities in gene identification, biological database searching, conceptual vector design, plant transformation workflows, CRISPR awareness, biosafety, and research-report preparation.
Program Highlights
• Introduction to C3 and C4 photosynthesis
• Understanding of C4 genes, enzymes, and pathways
• Basics of recombinant DNA technology
• Plant-vector and promoter-selection concepts
• Exposure to plant transformation and gene stacking
• Case studies on C4 rice and wheat
• Introduction to CRISPR and synthetic biology
• Use of plant genomics databases
• Research proposal and report preparation
• e-Certification + e-Marksheet
Course Curriculum
Module 1: C3 and C4 Photosynthesis
- Basics of photosynthesis and carbon fixation
- C3, C4, and CAM pathways
- Photorespiration
- Kranz anatomy
- Mesophyll and bundle-sheath cells
- Water-use and nitrogen-use efficiency
- Importance of C4 engineering in crops
Module 2: Molecular and Genetic Framework
- Major C4 genes and enzymes
- PEPC, PPDK, NADP-malic enzyme, and carbonic anhydrase
- RuBisCO and RuBisCO activase
- Promoters, terminators, and selectable markers
- Gene and protein sequence searching
- NCBI, BLAST, GenBank, and UniProt
Module 3: rDNA Technology and Plant Transformation
- Gene cloning and recombinant DNA concepts
- Plant-expression vectors
- Binary vectors and Ti plasmids
- Gene stacking and multigene expression
- Agrobacterium-mediated transformation
- Particle bombardment awareness
- PCR and gene-expression validation
- Conceptual vector-design activity
Module 4: C4 Crop Engineering and Biosafety
- Engineering C4 traits in C3 crops
- C4 rice and wheat case studies
- CRISPR-Cas and synthetic biology
- Biosafety and environmental risk
- GMO regulations and field trials
- Research proposal and internship-report preparation
Tools and Platforms Covered
NCBI
GenBank
BLAST
UniProt
Ensembl Plants
Gramene
KEGG
Plant Reactome
Google Scholar
PubMed
Google Colab
Python
Excel
Google Sheets
GenBank
BLAST
UniProt
Ensembl Plants
Gramene
KEGG
Plant Reactome
Google Scholar
PubMed
Google Colab
Python
Excel
Google Sheets
Real-World Applications
- Identification of C4 genes and enzymes
- Comparison of C3 and C4 pathways
- Plant gene and protein sequence analysis
- Conceptual plant-vector design
- Crop-transformation workflow development
- CRISPR-based crop-improvement awareness
- Biosafety and GMO assessment
- Plant biotechnology research and thesis support
- Climate-resilient crop-development studies
Who Should Attend & Prerequisites
- Biotechnology, botany, agriculture, and plant science students
- Genetics, genomics, molecular biology, and biochemistry learners
- Undergraduate and postgraduate students
- PhD scholars and researchers
- Faculty members
- Plant-breeding and tissue-culture professionals
- Bioinformatics beginners
- Learners interested in crop biotechnology and genetic engineering
Prerequisites: Basic knowledge of biology, genetics, molecular biology, or plant physiology is recommended. Coding experience is not mandatory.
Frequently Asked Questions
1. What is the Development of C4 Plants Using rDNA Technology Internship about?
The internship focuses on C3 and C4 photosynthesis, recombinant DNA technology, plant genetic engineering, vector design, crop transformation, CRISPR concepts, biosafety, and climate-resilient crop development.
2. Is this internship suitable for beginners?
Yes. The internship introduces key concepts in a simple, structured, and progressive manner, making it suitable for beginners as well as learners with a basic background in plant science or molecular biology.
3. Will practical activities be included?
Yes. Guided activities may include gene searching, sequence retrieval, database exploration, conceptual vector design, literature review, pathway comparison, and crop-engineering workflow preparation.
4. Is laboratory access required?
No. This online internship primarily focuses on computational analysis, conceptual experimental design, biological databases, research interpretation, and report preparation.
5. Is coding knowledge required?
No. Coding experience is not mandatory. Basic guidance for Google Colab, Python, spreadsheets, and database-based activities will be provided wherever required.
6. Which tools and databases will be covered?
Learners will be introduced to NCBI, GenBank, BLAST, UniProt, Ensembl Plants, Gramene, KEGG, Plant Reactome, Google Scholar, PubMed, Google Colab, Python, Excel, and Google Sheets.
7. What crops are discussed in the internship?
The internship includes examples and case studies related to introducing C4-related traits into C3 crops, with particular attention to research on C4 rice and wheat.
8. Will CRISPR and synthetic biology be covered?
Yes. Learners will receive an introductory understanding of CRISPR-Cas systems, synthetic biology, multigene expression, and their potential role in crop improvement.
9. Will I prepare a project or report?
Yes. The internship includes guidance on literature review, research-proposal preparation, conceptual experimental planning, and internship-report development.
10. Will I receive a certificate after completion?
Yes. Participants will receive an e-Certification + e-Marksheet after successfully completing the internship requirements.
The Development of C4 Plants Using rDNA Technology Internship provides a strong foundation in plant biotechnology, recombinant DNA technology, crop genetic engineering, bioinformatics, and climate-resilient agriculture. Through structured online learning, guided activities, and research-report preparation, participants can build practical knowledge for academic projects, research, and future careers in plant science and biotechnology.
