About the Bacterial Comparative Genomics Course
Program Highlights
Course Curriculum
Module 1: Environment Setup & Raw Data Processing
- Install and configure genomics tools using Conda package management
- Obtain and manage raw sequencing reads from public repositories
- Perform quality control assessment and adapter trimming of raw data
- Execute de novo genome assembly using SPAdes assembler
Module 2: Genome Refinement & Annotation
- Polish draft assemblies to correct errors and improve consensus accuracy using Pilon
- Reorder and scaffold contigs against reference genomes using RagTag
- Perform multi-locus sequence typing for strain identification and epidemiology
- Annotate draft genomes comprehensively using Prokka pipeline
Module 3: Functional & Comparative Genomics Analysis
- Identify antimicrobial resistance genes using Abricate screening tool
- Conduct pangenome analysis to reveal core and accessory genome dynamics
- Explore comparative genomics webservers including BV-BRC for integrated analysis
Module 4: Evolutionary Dynamics & Phylogenomics
- Reconstruct phylogenomic relationships from whole-genome comparisons
- Analyze genetic diversity and population structure across bacterial isolates
- Interpret horizontal gene transfer events and mobile genetic elements
Module 5: Antimicrobial Resistance & Virulence Profiling
- Characterize resistance mechanisms using curated antimicrobial databases
- Predict virulence factors and pathogenicity islands from genomic data
- Correlate genotype with phenotype for clinical and environmental isolates
Module 6: Pangenome Construction & Visualization
- Build pangenome matrices using Roary and related pangenome tools
- Visualize pangenome dynamics through interactive graphical representations
- Quantify gene presence-absence patterns and their biological significance
Module 7: Web-Based Comparative Genomics Platforms
- Navigate BV-BRC comprehensive bacterial bioinformatics resource center
- Integrate multi-omics data for systems-level bacterial analysis
- Leverage cloud-based infrastructure for large-scale comparative studies
Tools, Techniques, or Platforms Covered
SPAdes
Pilon
RagTag
Prokka
MLST
Abricate
Roary
BV-BRC
WSL2
Real-World Applications
- Apply Bacterial Comparative Genomics skills directly to academic research, thesis work, and publications
- Build a professional portfolio showcasing practical Bioinformatics competencies
- Solve industry-relevant problems using Bacterial Comparative Genomics methodologies and tools
- Contribute to open-source projects and collaborative research in Bioinformatics
- Prepare for competitive examinations, interviews, and professional certifications in Bioinformatics
Who Should Attend & Prerequisites
- Industry-recognized e-Certification + e-Marksheet from NSTC
- Hands-on training with practical projects and industrial datasets
- Dedicated expert mentorship and doubt resolution
Prerequisites:







