Zinc Finger Nucleases and TALENs: Alternatives to CRISPR

Master the Precision of Gene Editing with ZFNs and TALENs—Beyond CRISPR.

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MODE
Online/ e-LMS

TYPE
Self Paced

LEVEL
Moderate

DURATION
1 Month

About

Gene editing technologies have revolutionized the fields of genetics and molecular biology. While CRISPR is widely known, Zinc Finger Nucleases (ZFNs) and TALENs remain potent alternatives, offering unique advantages in terms of specificity and precision. This 1-month program explores the intricacies of ZFNs and TALENs, highlighting their molecular mechanisms, design strategies, and applications in various biological contexts. Participants will delve into the scientific underpinnings that make these technologies powerful tools in gene editing.
The program will cover the history, evolution, and comparison of ZFNs and TALENs with CRISPR, focusing on their unique advantages, such as targeting flexibility and reduced off-target effects. Additionally, participants will learn to design experiments utilizing these nucleases and explore real-world applications in fields such as functional genomics, agriculture, and therapeutic interventions.

Aim

This program aims to provide a comprehensive understanding of Zinc Finger Nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs), exploring their mechanisms, applications, and roles as alternatives to CRISPR technology. Participants will gain insights into the molecular tools used for gene editing, their advantages, and how they are applied in research and therapeutic development.

Program Objectives

Understand the mechanisms of Zinc Finger Nucleases (ZFNs) and TALENs.
Compare ZFNs and TALENs with CRISPR technologies.
Design and analyze gene-editing experiments using ZFNs and TALENs.
Explore real-world applications of these technologies in biotechnology and medicine.
Evaluate the ethical and regulatory aspects of gene-editing technologies.

Program Structure

Week 1: Introduction to Zinc Finger Nucleases and TALENs

Overview of Gene Editing Technologies: CRISPR, ZFNs, and TALENs.
Molecular Mechanisms of Zinc Finger Nucleases.
TALENs: Structure and Function.
Designing Zinc Finger Nucleases and TALENs for Gene Editing.

Week 2: Applications in Biotechnology

ZFNs and TALENs in Functional Genomics: Use Cases in Research.
Therapeutic Applications: Gene Therapy with ZFNs and TALENs.
Agricultural Biotechnology: Improving Crops through Gene Editing.
Ethical Considerations in the Use of Gene-Editing Tools.

Week 3: Bioinformatics and Experiment Design

Bioinformatics Tools for Target Selection and Analysis.
Designing Gene Editing Experiments with ZFNs and TALENs.
Data Management and Analysis in Gene Editing Studies.
Comparative Study: CRISPR vs. ZFNs vs. TALENs.

Week 4: Future Trends and Real-World Case Studies

Innovations in Gene Editing: The Next Generation of ZFNs and TALENs.
Case Studies: Successful Applications of ZFNs and TALENs in Research.
Preparing for Careers in Gene Editing: Skills and Knowledge Required.
Future of Gene Editing Technologies and Their Impact on Society.

Participant’s Eligibility

Undergraduate degree in Molecular Biology, Genetics, Biotechnology, or related fields.
Professionals in biomedical research or genetic engineering.
Individuals with a keen interest in alternative gene-editing technologies.

Program Outcomes

Proficiency in Zinc Finger Nucleases (ZFNs) and TALENs mechanisms.
Ability to design gene-editing experiments.
Comparative analysis between CRISPR, ZFNs, and TALENs.
Practical applications in medicine and agriculture.
Understanding of bioinformatics tools for gene editing analysis.

Fee Structure

Standard Fee: INR ~~4,998~~ USD ~~110~~

Discounted Fee: INR 2499 USD 55

We are excited to announce that we now accept payments in over 20 global currencies, in addition to USD. Check out our list to see if your preferred currency is supported. Enjoy the convenience and flexibility of paying in your local currency!

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Key Takeaways

Program Assessment

Certification to this program will be based on the evaluation of following assignment (s)/ examinations:

Exam	Weightage
Mid Term Assignments	50 %
Project Report Submission (Includes Mandatory Paper Publication)	50 %

To study the printed/online course material, submit and clear, the mid term assignments, project work/research study (in completion of project work/research study, a final report must be submitted) and the online examination, you are allotted a 1-month period. You will be awarded a certificate, only after successful completion/ and clearance of all the aforesaid assignment(s) and examinations.