• Home
  • /
  • siRNA-Based Gene Silencing
Biotechnology · Moderate · Video-based learning

siRNA-Based Gene Silencing

“Silence Genes. Amplify Precision. Master siRNA Today.”

Access 24/7 · 365 daysVideo lectures of 2+ hrsCertificate
Registration open

About This Course

Small interfering RNAs (siRNAs) have transformed molecular biology by offering precise and effective gene silencing solutions. This 2-day hands-on workshop dives into the foundational principles of RNA interference (RNAi) and guides participants through the process of understanding, designing, and analyzing siRNA molecules using leading bioinformatics tools.
The program is structured to provide clarity on the distinctions between siRNA, miRNA, and shRNA, while introducing RNA secondary structures and prediction tools like RNAfold, Mfold, and RNAstructure. Participants will also gain practical experience with ΔG analysis, structure ensemble generation, and siRNA design using Genscript. This course bridges the gap between theory and application, preparing attendees for careers in molecular biology, therapeutics, and RNA-based drug design.

Aim

To equip participants with a comprehensive understanding of RNA interference mechanisms, siRNA biology, and the computational tools used to design effective siRNA molecules for gene silencing in therapeutics and research.

Course Objectives

  • Understand the molecular basis of RNA interference and siRNA.
  • Differentiate between siRNA, miRNA, and shRNA and their applications.
  • Analyze RNA secondary structures using bioinformatics tools.
  • Gain hands-on experience with ΔG interpretation and RNA folding.
  • Learn practical siRNA design using Genscript and evaluate for efficiency.
Next: Syllabus →

Syllabus

Module 1: siRNA and RNA Structure Fundamentals

  • Introduction to RNAi and siRNA
  • siRNA vs miRNA vs shRNA
  • Applications in therapeutics and gene regulation
  • siRNA structure and functional rules
  • Off-target effects and GC content
  • Introduction to RNA secondary structures
  • RNA folding: base pairing, stem-loops, hairpins
  • RNA structure formats

Module 2: Tools and Hands-On Design

  • RNA structure prediction tools:
    RNAfold (ViennaRNA), Mfold, RNAstructure
  • Hands-on:
    Free energy (ΔG) interpretation
    Structure ensemble generation
  • Genscript – siRNA design
  • Hands-on siRNA sequence design

Who Should Enrol?

  • B.Tech / B.Sc / M.Sc / M.Tech graduates or final-year students from Biotechnology, Bioinformatics, Life Sciences, or allied disciplines
  • Researchers and industry professionals working in Genomics, Molecular Biology, Pharmaceutical R&D, or related areas
  • Anyone interested in Transcriptomics, Computational Biology, or exploring careers in bioinformatics and RNA-based technologies

Course Outcomes

  • Solid foundation in RNAi and gene silencing
  • Hands-on expertise with top RNA structure prediction tools
  • Capability to design effective siRNAs with reduced off-target effects
  • Enhanced understanding of RNA secondary structure and folding
  • Confidence to pursue research or job roles in RNA-based biotech
Next: Fees →

Course fee

Learners in India

₹2,499

Learners outside India

$50

  • Video lectures
  • Study material
  • Certificate after completion
  • 365-day access

Enrol in the course

The recordings are being uploaded. They appear in your dashboard as soon as they are ready, and we will e-mail you then.

Next: FAQ →

Frequently asked questions

How is this course taught?

Through recorded video lectures from the original live workshop, with the study material. Watch at your own pace, any time.

How long do I have access?

365 days from your registration, on your NanoSchool dashboard.

Do I get a certificate?

Yes, an e-certificate after completion, with a QR code anyone can verify and an "Add to LinkedIn" button.

How can I pay?

By card, UPI or PayPal on the payment page; international bank transfer is also available there.

What is the refund policy?

See our Returns, Refunds & Cancellation policy: Returns, Refunds & Cancellation policy

Need Help?

We’re here for you!


(+91) 120-4781-217
WhatsApp +91 99581 61117

Registration open

More upcoming workshops

Live, mentor-led and certified. Dates and times in IST.

See all workshops
Support