RNA Seq Differential Expression Analysis
Decode the Transcriptome: From Raw Reads to Functional Insights in Just 3 Days!
About This Course
RNA sequencing (RNA-Seq) has revolutionized transcriptomics by enabling researchers to study gene expression comprehensively and quantitatively. This workshop offers an in-depth introduction to RNA-Seq data analysis, particularly focusing on Differential Gene Expression (DGE) workflows using real-life datasets and open-source bioinformatics tools.
Through this interactive training, participants will gain practical skills in sequence data formats, quality checks, read mapping, count normalization, and expression profiling. They will also explore advanced visualization tools such as heat maps and volcano plots, culminating with functional enrichment analysis for biological interpretation. No prior coding experience is required, but familiarity with basic molecular biology is beneficial.
Aim
This 3-day workshop aims to provide hands-on understanding and theoretical foundations in RNA-Seq analysis, from raw data to biological interpretation. Participants will learn key workflows including data acquisition, quality control, mapping, normalization, differential expression analysis, and visualization techniques using R and DESeq2.
Course Objectives
-
Understand the RNA-Seq workflow from sequencing to biological inference
-
Learn to perform sequence quality control and read alignment
-
Explore normalization techniques and statistical analysis using DESeq2
-
Visualize results with heatmaps, volcano plots, and pathway analysis
-
Gain confidence in interpreting and presenting differential expression data
Syllabus
Module 1:
- RNA seq and DGE analysis
- Obtaining Data, Data formats
- Sequence quality Check
Module 2:
- Mapping and Quantification of Reads
- Count Normalization
Module 3:
- Differential gene expression analysis DESeq2
- Heat Map and Volcano plot using R
- Enrichment analysis
Who Should Enrol?
-
B.Tech / B.Sc / M.Sc / M.Tech graduates or final-year students from Biotechnology, Bioinformatics, Life Sciences, or allied disciplines
-
Professionals and researchers working in Genomics, Molecular Biology, Pharmaceutical R&D, or related areas
-
Enthusiasts and learners interested in Transcriptomics, Computational Biology, or exploring careers in bioinformatics
Course Outcomes
-
Clear understanding of RNA-Seq experimental design and pipelines
-
Familiarity with common tools: FastQC, STAR, HTSeq, DESeq2
-
Hands-on practice with R-based visualizations
-
Competence in interpreting gene expression changes
-
Ability to run basic enrichment and pathway analysis
-
Readiness to apply skills in academic or industry settings
Course fee
Learners in India
₹2,499
Learners outside India
$50
- Video lectures
- Study material
- Certificate after completion
- 365-day access
The recordings are being uploaded. They appear in your dashboard as soon as they are ready, and we will e-mail you then.
Frequently asked questions
How is this course taught?
Through recorded video lectures from the original live workshop, with the study material. Watch at your own pace, any time.
How long do I have access?
365 days from your registration, on your NanoSchool dashboard.
Do I get a certificate?
Yes, an e-certificate after completion, with a QR code anyone can verify and an "Add to LinkedIn" button.
How can I pay?
By card, UPI or PayPal on the payment page; international bank transfer is also available there.
What is the refund policy?
See our Returns, Refunds & Cancellation policy: Returns, Refunds & Cancellation policy
Registration open
More upcoming workshops
Live, mentor-led and certified. Dates and times in IST.
Mitochondrial Genomics & Disease: mtDNA Variant Analysis and Precision Medicine
- Dates
- 10–12 Nov 2026
- Time
- 5:30 PM IST
- Duration
- 3 Days
- Level
- Moderate
- Student₹2,499
- Ph.D. Scholar / Researcher₹3,499
- Academician / Faculty₹4,999
- Industry Professional₹5,999
AI-Powered Metabolomics & Precision Medicine: Disease Biomarker Discovery, Multi-Omics Analysis & Machine Learning
- Dates
- 12–14 Nov 2026
- Time
- 5:30 PM IST
- Duration
- 3 Days
- Level
- Moderate
- Student₹2,299
- Ph.D. Scholar / Researcher₹3,399
- Academician / Faculty₹4,499
- Industry Professional₹5,999
Hands-On miRNA & lncRNA Bioinformatics: Target Prediction, Disease Networks & Biomarker Discovery
- Dates
- 12–14 Nov 2026
- Time
- 5:30 PM IST
- Duration
- 3 Days
- Level
- Moderate
- Student₹2,499
- Ph.D. Scholar / Researcher₹3,499
- Academician / Faculty₹4,999
- Industry Professional₹6,299
Computational Biology, Systems Biology and Biomarker Discovery: Practical Applications in Disease Research and Nanomedicine
with Abhimanyu
- Dates
- 12–14 Nov 2026
- Time
- 5:30 PM IST
- Duration
- 3 Days
- Level
- Moderate
- Student₹2,199
- Ph.D. Scholar / Researcher₹3,199
- Academician / Faculty₹4,499
- Industry Professional₹5,999
Gut Microbiome Multi-Omics: Shotgun Metagenomics, Metabolomics & Precision Probiotics
- Dates
- 12–14 Nov 2026
- Time
- 5:00 PM IST
- Duration
- 3 Days
- Level
- Advanced
- Student₹2,499
- Ph.D. Scholar / Researcher₹3,499
- Academician / Faculty₹4,499
- Industry Professional₹6,499
Next-Generation Microbiome Therapeutics: AI, NGS & Python-Based Analysis
- Dates
- 12–15 Nov 2026
- Time
- 5:00 PM IST
- Duration
- 4 Days
- Level
- Moderate
- Student₹2,499
- Ph.D. Scholar / Researcher₹3,499
- Academician / Faculty₹4,499
- Industry Professional₹6,499






