iPSC, Organoids, Microphysiology & Organ-on-Chip: Experimental Design, Validation & Drug Testing
From Stem Cells to Human-Relevant Models: Design, Validate and Interpret iPSC, Organoid & Organ-on-Chip Experiments
About This Course
This 3-day live virtual workshop introduces advanced human-relevant in-vitro models used in disease modeling, drug discovery, toxicology and translational research.
Participants will learn how iPSC-derived models progress from 2D cell systems to spheroids, organoids and dynamic microphysiological/organ-on-chip platforms. Guided hands-on cases will help participants evaluate model quality, analyze experimental data and design reliable follow-up experiments.
Aim
To provide participants with a practical understanding of iPSC, spheroid, organoid, microphysiology and organ-on-chip experimental workflows, with emphasis on experimental design, quality control, validation, troubleshooting and drug-response interpretation.
Workshop Objectives
- Understand iPSC culture, differentiation and quality-control workflows
- Design healthy, disease and isogenic experimental groups
- Understand spheroid and organoid development and maturation
- Evaluate morphology, viability and molecular-marker data
- Understand tissue microphysiology, barriers, gradients and mechanical forces
- Design MPS and organ-on-chip experiments
- Interpret TEER, permeability, viability and biomarker readouts
- Identify experimental failures, variability and technical artefacts
- Compare 2D, organoid and MPS models for different research applications
- Apply reproducibility and validation principles to experimental studies
Workshop Structure
Day 1: iPSC Experimental Design & Quality Control
- iPSC workflow and research applications
- Culture systems, matrices and passaging
- Morphology, pluripotency and genomic QC
- Healthy, disease and isogenic controls
- Replicates, batch effects and experimental endpoints
- Common culture failures and troubleshooting
Hands-On: Evaluate iPSC morphology and marker data; design an iPSC disease-model experiment.
Tools: Google Colab, microscopy images, iPSC QC dataset
Day 2: Spheroid & Organoid Development and Validation
- 2D vs spheroid vs organoid models
- Spheroid generation and culture parameters
- Organoid differentiation, organization and maturation
- Tissue-specific organoid models
- Morphology, viability and marker-based validation
- Variability, reproducibility and experimental failure
Hands-On: Analyze spheroid/organoid images and validation data; classify successful and failed conditions.
Tools: Fiji/ImageJ, microscopy dataset, organoid QC data
Day 3: Microphysiology, MPS & Organ-on-Chip Drug Testing
- Fundamentals of human tissue microphysiology
- Cell–cell, tissue–tissue and microenvironment interactions
- Physiological gradients, barriers, mechanical forces and fluid flow
- Microphysiological Systems (MPS) and organ-on-chip principles
- Chip configuration, perfusion and shear-stress parameters
- TEER, permeability, viability and biomarker readouts
- Drug efficacy, toxicity and translational applications
- MPS validation, troubleshooting and reproducibility
Hands-On: Analyze a virtual MPS/organ-on-chip drug-response experiment and design an optimized study.
Tools: Google Colab, Python, pandas, Matplotlib
Who Should Enrol?
- Undergraduate & Postgraduate Students — Biotechnology, Life Sciences, Biomedical Sciences, Bioengineering, Pharmacy and related disciplines
- PhD Scholars & Researchers — Stem cells, tissue engineering, organoids, toxicology, pharmacology and disease modeling
- Faculty & Academicians — Teaching or conducting research in advanced cell and tissue models
- Pharma & Biotechnology Professionals — Drug discovery, preclinical research, assay development and translational biology
- CRO & R&D Professionals — Human-relevant models, toxicology and preclinical testing
Important Dates
Registration Ends
August 31, 2026
IST 4:30 PM
Workshop Dates
August 31, 2026 – September 2, 2026
IST 5:00 PM
Workshop Outcomes
- Design an iPSC-based disease-model experiment
- Evaluate iPSC quality and experimental suitability
- Assess spheroid and organoid development and validation
- Interpret microscopy, viability and marker-expression results
- Explain key principles of human tissue microphysiology
- Understand flow, shear stress and barrier functions in MPS
- Analyze organ-on-chip drug-response datasets
- Distinguish biological effects from technical problems
- Select appropriate controls, replicates and experimental endpoints
- Decide whether an experiment should proceed, be optimized or be repeated
- Design an improved follow-up experiment based on the results
Fee Structure
Student Fee
₹1999 | $65
Ph.D. Scholar / Researcher Fee
₹2999 | $75
Academician / Faculty Fee
₹3999 | $85
Industry Professional Fee
₹5999 | $105
What You’ll Gain
- Live & recorded sessions
- e-Certificate upon completion
- Post-workshop query support
- Hands-on learning experience
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