AI-Enabled RNA Therapeutics and Exosome-Based Targeted Drug Delivery

To provide participants with a research-oriented understanding of AI-enabled RNA therapeutic design and exosome-based targeted drug delivery, with emphasis on RNA modality selection, extracellular vesicle engineering, therapeutic workflow planning, and translational biomedical applications.

• To introduce the fundamentals of RNA therapeutics and nucleic acid-based medicine.
• To explain key RNA modalities such as siRNA, ASO, circRNA, saRNA, miRNA, and mRNA.
• To understand the role of exosomes and extracellular vesicles in targeted drug delivery.
• To explore AI-assisted approaches for target identification, literature mining, and therapeutic workflow planning.
• To demonstrate RNA sequence analysis, RNA folding, and secondary structure interpretation.
• To analyze exosome-based cargo loading, source selection, and targeting strategies.
• To connect RNA therapeutic design with exosome-mediated delivery for precision medicine applications.
• To discuss translational, clinical, regulatory, and safety challenges in RNA-exosome therapeutics.
• To help participants develop research-oriented workflows for cancer therapy, genetic disorders, rare diseases, and biomedical applications.

📅 Day 1: RNA Therapeutics & AI-Assisted Design

• Understanding RNA-based therapeutics and nucleic acid medicine platforms for modern biomedical applications
• Overview of major RNA therapeutic modalities including siRNA, ASO, circRNA, saRNA, miRNA, and mRNA
• Applications of RNA therapeutics in cancer, genetic disorders, viral infections, rare diseases, and precision medicine
• Key challenges in RNA therapeutics including stability, degradation, immune response, off-target effects, and delivery barriers
• AI-assisted target identification, therapeutic candidate screening, and biomedical literature mining
• RNA sequence analysis, secondary structure prediction, and interpretation of therapeutic relevance
• Design considerations for RNA therapeutic optimization and translational development
• Skills gained: RNA therapeutic target analysis, RNA modality selection, literature-based screening, and AI-supported research interpretation
• Tools covered: PubMed, NCBI Gene, RNAcentral, miRBase, RNAfold / ViennaRNA, ChatGPT / Gemini

Hands-on Activity:

• AI-Assisted RNA Therapeutic Target Analysis: Search disease-associated RNA targets using biomedical databases, screen published RNA-based studies, perform basic RNA sequence interpretation, and explore RNA folding and secondary structure prediction workflows

📅 Day 2: Exosome Engineering & Targeted Drug Delivery

• Introduction to exosomes and extracellular vesicles as biological nano-carriers for therapeutic delivery
• Understanding EV biogenesis, cargo transport, and intercellular communication in disease and therapy
• Exosome-mediated delivery of RNA, drugs, proteins, biologics, and advanced therapeutic molecules
• Cargo loading strategies for RNA molecules and therapeutic compounds in exosome-based systems
• Surface engineering and ligand-based targeting approaches for tissue-specific therapeutic delivery
• Applications of exosome delivery in cancer therapy, inflammatory diseases, genetic disorders, regenerative medicine, and precision medicine
• Challenges in EV isolation, purification, characterization, scalability, safety, and clinical translation
• AI applications in exosome cargo prediction, targeting efficiency, delivery optimization, and therapeutic planning
• Skills gained: exosome database exploration, cargo-target mapping, delivery strategy planning, and AI-supported exosome design logic
• Tools covered: ExoCarta, Vesiclepedia, EV-TRACK, PubMed, Google Scholar, BioRender, ChatGPT / Gemini

Hands-on Activity:

• Exosome-Based Therapeutic Delivery Strategy Design: Explore exosome and extracellular vesicle databases, analyze exosome-mediated RNA delivery studies, select exosome source, therapeutic cargo, and target tissue, and develop a conceptual exosome-based RNA or drug delivery strategy

📅 Day 3: Integrated RNA-Exosome Therapeutic Workflow

• Developing an end-to-end RNA-exosome therapeutic workflow for targeted biomedical applications
• Disease target selection, RNA candidate prioritization, and therapeutic relevance assessment
• Selection of suitable RNA modality based on disease mechanism, target biology, and therapeutic objective
• Exosome source selection for targeted RNA, drug, protein, and biologic delivery applications
• Target tissue and cell-type mapping for precision delivery planning and translational workflow design
• AI-guided prediction of RNA stability, toxicity, delivery efficiency, and therapeutic relevance
• Integration of RNA therapeutic design with exosome-based delivery strategies for advanced nanomedicine
• Clinical, manufacturing, scalability, safety, and regulatory considerations for RNA-exosome therapeutics
• Future scope of RNA therapeutics, exosome medicine, nanomedicine, and precision delivery systems
• Skills gained: integrated therapeutic workflow design, RNA-exosome strategy development, disease-target mapping, and translational research planning
• Tools covered: Google Colab, PubMed, NCBI, ExoCarta, Vesiclepedia, RNAcentral, miRBase, RNAfold / ViennaRNA, BioRender, ChatGPT / Gemini

Hands-on Activity:

• AI-Supported RNA-Exosome Therapeutic Workflow Design: Select a disease model, RNA modality, exosome source, and target tissue, then build a conceptual RNA-exosome delivery strategy with key translational challenges and therapeutic relevance

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• Open to students, researchers, faculty members, and industry professionals from life sciences, biotechnology, and biomedical domains.
• Suitable for learners from Biotechnology, Bioinformatics, Molecular Biology, Genetics, Microbiology, Life Sciences, and Biochemistry.
• Relevant for participants from Pharmaceutical Sciences, Biomedical Engineering, Nanotechnology, Computational Biology, Drug Discovery, and Translational Medicine.
• Basic understanding of molecular biology, biotechnology, or biomedical sciences is recommended.
• Prior coding experience is not mandatory.

• Understand key concepts in RNA therapeutics and nucleic acid-based medicine.
• Differentiate major RNA modalities including siRNA, ASO, circRNA, saRNA, miRNA, and mRNA.
• Explain the role of exosomes and extracellular vesicles in targeted therapeutic delivery.
• Apply AI-assisted approaches for literature mining, target identification, and workflow planning.
• Perform basic RNA sequence analysis and secondary structure interpretation.
• Analyze exosome-based cargo delivery and targeting strategies.
• Design a conceptual RNA-exosome therapeutic delivery workflow.
• Understand translational, safety, clinical, and regulatory challenges in RNA-exosome therapeutics.
• Develop research-oriented ideas for academic projects, proposals, and publications.