New Year Offer End Date: 30th April 2024
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Program

Next-Generation Sequencing Data Analysis using Galaxy Platform

Next-Generation Sequencing (NGS), Galaxy Platform, Bioinformatics, Genomics, Molecular Biology, Differential gene expression, NGS pipelines, Bioinformatic tools

About Program:

The Next-Generation Sequencing Data Analysis using the Galaxy Platform program is designed to address the growing need for accessible and efficient tools for analyzing complex genomic data. By offering comprehensive training modules and hands-on workshops, the program aims to equip researchers and bioinformatics professionals with the knowledge and skills required to navigate the Galaxy bioinformatics platform effectively. Participants will gain a deep understanding of Galaxy’s user-friendly interface, diverse toolset, and workflow capabilities, allowing them to confidently preprocess, align, and analyze NGS data for various research applications. Through practical exercises and real-world examples, the program fosters proficiency in data preprocessing, variant calling, and downstream analysis, empowering participants to leverage Galaxy’s capabilities for genomics research, personalized medicine, and other life sciences endeavors.

Aim: The aim of the Next-Generation Sequencing Data Analysis using the Galaxy Platform program is to empower researchers and bioinformatics professionals with the knowledge and skills required to effectively analyze next-generation sequencing (NGS) data using the Galaxy bioinformatics platform. Through comprehensive training modules and hands-on workshops, the program aims to familiarize participants with Galaxy’s user-friendly interface, diverse toolset, and workflow capabilities for processing, analyzing, and interpreting NGS data. By providing practical training in data preprocessing, alignment, variant calling, and downstream analysis, the program enables participants to harness the power of Galaxy for genomics research, personalized medicine, and other applications in the life sciences.

Program Objectives:

  • Understand the principles and concepts of NGS data analysis.
  • Familiarize participants with the Galaxy Platform and its tools for NGS data analysis.
  • Gain hands-on experience in executing NGS data analysis workflows using Galaxy.
  • Learn to perform quality control, read mapping, variant calling, and gene expression analysis using NGS data.
  • Explore pathway analysis techniques and interpret the results.
  • Develop the ability to construct and customize analysis pipelines in Galaxy.
  • Enhance skillsDay1 in interpreting and visualizing NGS data analysis results.
  • Learn best practices and optimization strategies for efficient NGS data analysis.

What you will learn?

Day 1

  • Introduction to NGS and galaxy platform
  • Galaxy tools for genome assembly
  • Galaxy tools for genome annotation
  • Identification of Variants

Day 2

  • RNA-seq analysis using Galaxy tools.
  • Transcriptome assembly using galaxy tools like Hisat2 followed by Feature count
  • Differential gene expression analysis using DESeq2

Students require to have good laptop with high-speed internet connection and They can create a user account in Galaxy portal (https://usegalaxy.org) using their academic email id.

Fee Plan

INR 1999 /- OR USD 50

Intended For :

  1. Educational Background: Participants should have a background in biology, bioinformatics, computational biology, genetics, or a related field. This ensures that they have the foundational knowledge required to understand genomic data analysis concepts.
  2. Proficiency in Bioinformatics Tools: While prior experience with Galaxy is not always necessary, participants should have basic proficiency in bioinformatics tools and concepts. Familiarity with next-generation sequencing data analysis pipelines, command-line interfaces, and relevant file formats (e.g., FASTQ, BAM) is beneficial.
  3. Computer Skills: Participants should be comfortable working with computers and software tools. Basic knowledge of operating systems (e.g., Linux, macOS, Windows) and file management is essential for navigating the Galaxy platform and analyzing NGS data.
  4. Motivation and Interest: Participants should demonstrate a strong interest in genomic research, bioinformatics, or related fields. They should be motivated to learn and apply new skills in NGS data analysis using the Galaxy platform to address research questions or advance their career goals.
  5. Access to Resources: Depending on the program format (e.g., online workshop, in-person training), participants may need access to a computer with internet connectivity to participate in hands-on exercises and access training materials. Access to a Galaxy server or cloud-based instance may also be required.
  6. Language Proficiency: If the program is conducted in a language other than the participant’s native language, proficiency in the language of instruction (e.g., English) may be necessary to understand lectures, instructions, and training materials effectively.
  7. Prerequisites: Some programs may have specific prerequisites, such as completion of introductory bioinformatics courses, familiarity with basic molecular biology concepts, or prior experience with genomic data analysis. Participants should review the program requirements and prerequisites before applying.

Career Supporting Skills

NGS Data Analysis Galaxy Platform Bioinformatics Genomics Molecular Biology Gene Expression

Program Outcomes

  • Proficiency in NGS data analysis techniques using the Galaxy Platform.
  • Improved understanding of bioinformatics principles and their application to NGS data analysis.
  • Ability to navigate and utilize the Galaxy Platform for NGS data analysis workflows.
  • Enhanced skills in quality control, read mapping, variant calling, and gene expression analysis using NGS data.
  • Knowledge of pathway analysis techniques and interpretation of pathway analysis results.
  • Competence in constructing and customizing analysis pipelines in the Galaxy Platform.
  • Proficiency in interpreting and visualizing NGS data analysis results.
  • Understanding of statistical analysis methods commonly used in NGS data analysis.
  • Familiarity with functional annotation and enrichment analysis of NGS data.
  • Development of troubleshooting skills to identify and resolve issues in NGS data analysis workflows.