RNA Interference (RNAi) and siRNA Techniques
Master the Science of RNA Interference and siRNA Techniques to Transform Gene Therapy and Genetic Research
Skills you will gain:
About Workshop:
RNA interference (RNAi) is a natural cellular process used to regulate gene expression. This 3-day workshop will delve into the fundamental mechanisms behind RNAi and its application in controlling the expression of specific genes. Participants will explore both theoretical and practical aspects of small interfering RNA (siRNA) techniques, from the molecular biology involved to real-world applications in research and medicine.
Throughout the workshop, we will focus on the role of RNAi in biological research, including gene function analysis, therapeutic approaches in diseases like cancer and viral infections, and gene editing techniques. The workshop will offer hands-on guidance in designing siRNA experiments, selecting appropriate delivery systems, and interpreting results, making it a valuable tool for molecular biologists, geneticists, and medical researchers.
Aim: This workshop aims to equip participants with a comprehensive understanding of RNA interference (RNAi) and small interfering RNA (siRNA) techniques. It will cover key mechanisms, applications in gene silencing, and how RNAi can be utilized in research and therapeutic strategies. By the end of the workshop, participants will be able to design and implement RNAi experiments for various biological studies.
Workshop Objectives:
- Understand the mechanisms of RNA interference and its biological significance.
- Learn about siRNA design and its applications in gene silencing.
- Explore delivery methods for siRNA in cellular and animal models.
- Analyze case studies of RNAi in therapeutic development.
- Develop skills in interpreting RNAi data and troubleshooting experiments.
What you will learn?
Day 1: Introduction to RNA Interference (RNAi)
Introduction to RNA Interference:
- What is RNAi?
- Historical background and discovery of RNAi (Fire and Mello’s work)
- Overview of gene silencing pathways
- Role of small RNAs in gene regulation
Mechanism of RNAi:
- Step-by-step mechanism (Dicer, RISC complex)
- Difference between siRNA and miRNA pathways
- Key proteins involved (Argonaute, Dicer)
Biological Functions of RNAi:
- Post-transcriptional gene silencing (PTGS)
- Role in defense against viruses and transposons
- Applications in gene expression regulation
Applications of RNAi in Research:
- Functional genomics and gene knockdown
- Use in model organisms (C. elegans, Drosophila, etc.)
- Discussion on therapeutics and drug development
Day 2: siRNA Techniques: Design and Delivery
siRNA Basics:
- What is siRNA? Structure and function
- Differences between siRNA and miRNA
- siRNA-mediated gene silencing
Designing siRNAs:
- Key criteria for designing siRNAs
- Tools and software for siRNA design (e.g., Invitrogen BLOCK-iT, siDirect)
- Avoiding off-target effects
- Selection of target sequences, sense and antisense strand considerations
siRNA Delivery Methods:
- Lipid-based delivery systems (Lipofectamine)
- Electroporation and viral vectors (AAV, lentivirus)
- Nanoparticles for siRNA delivery
- Challenges in siRNA delivery: Stability, off-target effects, cellular uptake
siRNA Knockdown Assays:
- Confirming siRNA efficiency
- Techniques: RT-qPCR, Western blotting, and immunofluorescence
Day 3: Applications of RNAi and siRNA in Research and Therapeutics
RNAi and Disease Research:
- Role in cancer, neurodegenerative diseases, and viral infections
- Case studies: siRNA therapies in clinical trials (Patisiran for ATTR amyloidosis)
Therapeutic Applications of siRNA:
- RNAi-based drugs: Development and FDA approval (Onpattro, Givlaari)
- Challenges in therapeutic siRNA delivery and stability
- Ongoing clinical trials using RNAi and siRNA
Gene Therapy and siRNA:
- Role of siRNA in gene therapy and personalized medicine
- Ethical considerations and regulatory frameworks
Future Directions in RNAi and siRNA Research:
- Next-generation RNAi technologies
- CRISPR vs RNAi in gene editing
Mentor Profile
Fee Plan
Important Dates
11 Dec 2024 Indian Standard Timing 2:00 PM
11 Dec 2024 to 13 Dec 2024 Indian Standard Timing 3:00 PM
Get an e-Certificate of Participation!
Intended For :
- Undergraduate or graduate degree in Biotechnology, Molecular Biology, Genetics, Biochemistry, or related fields.
- Professionals working in genetic research, biomedical fields, or pharmaceutical industries.
- Individuals interested in gene therapy, drug development, and molecular diagnostics.
Career Supporting Skills
Workshop Outcomes
- Proficiency in RNAi mechanisms and siRNA design.
- Practical understanding of RNAi applications in biomedical research.
- Knowledge of RNAi-based therapeutic developments.
- Ability to perform gene silencing experiments.
- Enhanced skills in molecular diagnostics using RNA technologies.
