CRISPR-Cas Genome Editing: Workflow, Tools and Techniques

Unlocking Genomic Potential: CRISPR-Cas at the Forefront of Precision Editing

About Course:

CRISPR-Cas gene editing technology is a powerful tool that has revolutionized the field of biology. It allows researchers to make precise and efficient changes to the genome of any organism. This has led to a rapid increase in our understanding of gene function and disease mechanisms, and it has also opened up new possibilities for gene therapy and other therapeutic applications.

In this three-day workshop, participants will learn about the basics of CRISPR-Cas gene editing, its advanced applications and ethical considerations. The workshop will include lectures, hands-on demonstrations, and discussions with experts in the field.

Aim: The workshop aims to provide participants with a comprehensive overview of CRISPR-Cas gene editing technology and its applications. The workshop will cover the basics of CRISPR-Cas, including how it works, different types of CRISPR-Cas systems, and its potential impact on various fields, such as basic research, translational research, and gene therapy.

Course Objectives:

Upon completion of the workshop, participants will be able to:

  • Understand the principles of CRISPR-Cas gene editing
  • Design and conduct CRISPR-Cas gene editing experiments
  • Analyze and interpret CRISPR-Cas gene editing results
  • Troubleshoot common CRISPR-Cas gene editing challenges
  • Apply CRISPR-Cas gene editing technology to their research

What you will learn?

Day 1: Introduction and applications of CRISPR-Cas

  • What is CRISPR-Cas?
  • How does CRISPR-Cas work?
  • Different types of CRISPR-Cas systems
  • CRISPR in Basic Research
  • CRISPR in Translational Research
  • CRISPR in Gene therapy

Day 2: CRISPR-Cas gene editing workflows and challenges and troubleshooting

  • Designing a CRISPR-Cas experiment
  • Assembling CRISPR-Cas components
  • Transfecting or transducing cells with CRISPR-Cas components
  • Validating gene editing results
  • Off-target effects
  • Low efficiency
  • Delivery challenges

Day 3: Advanced CRISPR-Cas gene editing techniques, ethics and regulation

  • CRISPR-Cas gene activation and repression
  • CRISPR-Cas gene editing in primary cells and tissues
  • CRISPR-Cas genome engineering for animal models
  • Responsible use of CRISPR-Cas
  • Intellectual property considerations
  • Regulatory landscape for CRISPR-Cas gene editing

Mentor Profile

Dr. Darshan Panda is a Senior Researcher in the Department of Crop Physiology & Biochemistry at the ICAR-National Rice Research Institute, Bidyadharpur, Cuttack. He received his Ph.D. Degree in Biotechnology from Ravenshaw University in 2022. He is currently working as a senior researcher at ICAR-NRRI, Cuttack. He has published his research findings through the publication of over 50 articles in esteemed academic international journals. He has 10 years of experience in the fields of plant physiology, molecular biology and biotechnology. His area of expertise includes crop photobiology, physiology, biochemistry, molecular biology, tissue culture and biotechnology. He is the author of two textbooks in biotechnology and microbiology.

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Fee Plan

INR 1999 /- OR USD 50

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Intended For : Genetics and Genomics Research, Biomedical and Clinical Genomics, Pharmaceuticals and Drug Development, Bioinformatics and Computational Biology, Population Genetics and Anthropology, Agricultural and Environmental Genetics, Forensic Genetics, Public Health and Epidemiology, Startups and Biotechnology Companies, Personalized Medicine and HealthTech, Veterinary Genetics

Career Supporting Skills

Gene Editing DesignMolecular Data AnalysisGene TherapyCell Transfection/TransductionAnimal Model Experience

Course Outcomes

  • A comprehensive understanding of CRISPR-Cas gene editing technology and its applications
  • The ability to design and conduct CRISPR-Cas gene editing experiments
  • The ability to analyze and interpret CRISPR-Cas gene editing results
  • The ability to troubleshoot common CRISPR-Cas gene editing challenges
  • The ability to apply CRISPR-Cas gene editing technology to your research