Day 1: Introduction and applications of CRISPR-Cas

What is CRISPR-Cas? How does CRISPR-Cas work? Different types of CRISPR-Cas systems CRISPR in Basic Research CRISPR in Translational Research CRISPR in Gene therapy

Day 2: CRISPR-Cas gene editing workflows and challenges and troubleshooting

Designing a CRISPR-Cas experiment Assembling CRISPR-Cas components Transfecting or transducing cells with CRISPR-Cas components Validating gene editing results Off-target effects Low efficiency Delivery challenges

Day 3: Advanced CRISPR-Cas gene editing techniques, ethics and regulation

CRISPR-Cas gene activation and repression CRISPR-Cas gene editing in primary cells and tissues CRISPR-Cas genome engineering for animal models Responsible use of CRISPR-Cas Intellectual property considerations Regulatory landscape for CRISPR-Cas gene editing